Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24107 | 5' | -53.1 | NC_005262.1 | + | 49828 | 0.67 | 0.796972 |
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Target: 5'- cGCgCGAUGGAGcgCgcgaauggCCCGGUGCCGGc -3' miRNA: 3'- -CGgGCUACUUUuaG--------GGGUCGCGGUUc -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 9148 | 0.67 | 0.796972 |
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Target: 5'- gGCCgGucgGAAugcgGAUCaCCCGGCGCCu-- -3' miRNA: 3'- -CGGgCua-CUU----UUAG-GGGUCGCGGuuc -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 21262 | 0.67 | 0.8254 |
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Target: 5'- aUCCGAgcggg--CUUCAGCGCCAAGa -3' miRNA: 3'- cGGGCUacuuuuaGGGGUCGCGGUUC- -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 48076 | 0.67 | 0.8254 |
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Target: 5'- uGCCgCGAcGAGccg-CCCGGCGCCGAc -3' miRNA: 3'- -CGG-GCUaCUUuuagGGGUCGCGGUUc -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 54192 | 0.66 | 0.865304 |
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Target: 5'- uGUCCGAgcgcgugCUCCAGCGCCc-- -3' miRNA: 3'- -CGGGCUacuuuuaGGGGUCGCGGuuc -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 44007 | 0.66 | 0.863672 |
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Target: 5'- cGCCCGAUGugcgacgaCCAcGCGCaCGAGa -3' miRNA: 3'- -CGGGCUACuuuuagg-GGU-CGCG-GUUC- -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 54645 | 0.66 | 0.842462 |
|
Target: 5'- cGCCgGGUGAuccgcauUCcgaccggCCCGGCGCCGGu -3' miRNA: 3'- -CGGgCUACUuuu----AG-------GGGUCGCGGUUc -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 3029 | 0.66 | 0.843339 |
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Target: 5'- uCCCGAagaUGAAGuucuucgagcgcGUCuCCCAGCcuucGCCGAGc -3' miRNA: 3'- cGGGCU---ACUUU------------UAG-GGGUCG----CGGUUC- -5' |
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| 24107 | 5' | -53.1 | NC_005262.1 | + | 45097 | 0.66 | 0.851975 |
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Target: 5'- cGCCUGgcGGAAGUCCgugCCAGCcgauauGCgCAAGg -3' miRNA: 3'- -CGGGCuaCUUUUAGG---GGUCG------CG-GUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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