Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24110 | 3' | -61.1 | NC_005263.2 | + | 35525 | 0.77 | 0.070555 |
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Target: 5'- cGCGcCGACUGcgcCCGGCGgCGcGCGGGCGGg -3' miRNA: 3'- -UGU-GCUGAU---GGCCGUgGC-CGCCCGUC- -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 27678 | 0.77 | 0.070752 |
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Target: 5'- aGCACGGCcgccuucuugugcgcGCCGGCGCCGGCGGccGCAu -3' miRNA: 3'- -UGUGCUGa--------------UGGCCGUGGCCGCC--CGUc -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 17393 | 0.78 | 0.056343 |
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Target: 5'- uGC-CGGCgcgGCCGcGCGCCuGGCGGGCGGu -3' miRNA: 3'- -UGuGCUGa--UGGC-CGUGG-CCGCCCGUC- -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 20410 | 0.79 | 0.048914 |
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Target: 5'- uAUGCGGCcGCCGGCGCCGGCGcGCAc -3' miRNA: 3'- -UGUGCUGaUGGCCGUGGCCGCcCGUc -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 48007 | 1.08 | 0.000261 |
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Target: 5'- gACACGACUACCGGCACCGGCGGGCAGg -3' miRNA: 3'- -UGUGCUGAUGGCCGUGGCCGCCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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