miRNA: 3'- -UGUGcUGauGGCCGUGGCCGCC-CGuc -5'
miRNA: 3'- ugUGCUGaUGGCCGUGgCCGcc-CGUc -5'
miRNA: 3'- -UGU-GCUGa--UGgCCG----------UGGCCGC-CCGUC- -5'
miRNA: 3'- -UG-UGCUGaugGCCGUGgCCGC--CCGUC- -5'
miRNA: 3'- -UGugCUgaUG-GCCGUGGCCg--CCCGUc -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24110 | 3' | -61.1 | NC_005263.2 | + | 18560 | 0.66 | 0.392185 |
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Target: 5'- cGCGCaGC--CCGGCACCGGUGGcGUc- -3' miRNA: 3'- -UGUGcUGauGGCCGUGGCCGCC-CGuc -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 24312 | 0.66 | 0.392185 |
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Target: 5'- -gACGGCcGCCGGCACgGGCaucGCAa -3' miRNA: 3'- ugUGCUGaUGGCCGUGgCCGcc-CGUc -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 20795 | 0.66 | 0.401042 |
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Target: 5'- gGCGcCGACgcaAUgGGUcaaguuccugGCCGGCGcGGCAGg -3' miRNA: 3'- -UGU-GCUGa--UGgCCG----------UGGCCGC-CCGUC- -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 24538 | 0.66 | 0.401042 |
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Target: 5'- gGCaACGGCgg-CGGCGCgGGCGgcGGCGGc -3' miRNA: 3'- -UG-UGCUGaugGCCGUGgCCGC--CCGUC- -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 21626 | 0.66 | 0.401042 |
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Target: 5'- gAUugGA--GCgCGGCGCCGGaauGGGCGa -3' miRNA: 3'- -UGugCUgaUG-GCCGUGGCCg--CCCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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