Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24110 | 3' | -61.1 | NC_005263.2 | + | 2668 | 0.69 | 0.258121 |
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Target: 5'- -gGCGAC-ACCGGCagccugcagugucgcGCCGGC-GGCGGc -3' miRNA: 3'- ugUGCUGaUGGCCG---------------UGGCCGcCCGUC- -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 2378 | 0.69 | 0.22872 |
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Target: 5'- gACGCGGCguaacccaugaCGGCGCCGGCGGucGCu- -3' miRNA: 3'- -UGUGCUGaug--------GCCGUGGCCGCC--CGuc -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 1932 | 0.77 | 0.063063 |
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Target: 5'- uGCACGAgCgcGCCGGCACCGGCGacgccGGCAa -3' miRNA: 3'- -UGUGCU-Ga-UGGCCGUGGCCGC-----CCGUc -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 1338 | 0.7 | 0.223532 |
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Target: 5'- aACACGAUcacgGCCGGUugcagaaucuuuuuGCCGGC-GGCGGc -3' miRNA: 3'- -UGUGCUGa---UGGCCG--------------UGGCCGcCCGUC- -5' |
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| 24110 | 3' | -61.1 | NC_005263.2 | + | 897 | 0.7 | 0.214007 |
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Target: 5'- aGCGCGGCggUCGcCGCCGGCGGGUu- -3' miRNA: 3'- -UGUGCUGauGGCcGUGGCCGCCCGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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