Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24110 | 5' | -53.7 | NC_005263.2 | + | 47343 | 0.66 | 0.765595 |
Target: 5'- gGCUGCaguggacaggCACCUaucaGGUGCuGcAGGGCGCa -3' miRNA: 3'- aUGAUGga--------GUGGA----CCAUG-C-UUCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 10004 | 0.66 | 0.765595 |
Target: 5'- -uCUugCgCGCCaGGUGCG--GGCGCa -3' miRNA: 3'- auGAugGaGUGGaCCAUGCuuCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 7211 | 0.66 | 0.755072 |
Target: 5'- cGCgcgACCaUCGCC-GGcgACcaGAAGGCGCg -3' miRNA: 3'- aUGa--UGG-AGUGGaCCa-UG--CUUCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 40604 | 0.66 | 0.727124 |
Target: 5'- cGC-ACCUCGCCgucagcGGUGCGcggcacuucgaggccAAGcGCGCa -3' miRNA: 3'- aUGaUGGAGUGGa-----CCAUGC---------------UUC-CGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 33720 | 0.67 | 0.711781 |
Target: 5'- aUGCUGCCgCACCcGG-ACGAGcGGCccgGCa -3' miRNA: 3'- -AUGAUGGaGUGGaCCaUGCUU-CCG---CG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 20344 | 0.67 | 0.655894 |
Target: 5'- -cCUGCCgccgCACCU----CGggGGCGCg -3' miRNA: 3'- auGAUGGa---GUGGAccauGCuuCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 30365 | 0.67 | 0.655894 |
Target: 5'- cGCUGCCgccggCGCC-GaGUGCGAGccccaugcggcGGCGCu -3' miRNA: 3'- aUGAUGGa----GUGGaC-CAUGCUU-----------CCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 4003 | 0.68 | 0.644601 |
Target: 5'- cUGCUGCgUgACCU----CGAAGGCGCa -3' miRNA: 3'- -AUGAUGgAgUGGAccauGCUUCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 42900 | 0.69 | 0.588166 |
Target: 5'- cGC-GCgCUCGCCUGGacGCGAGuGCGCg -3' miRNA: 3'- aUGaUG-GAGUGGACCa-UGCUUcCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 26837 | 0.69 | 0.565795 |
Target: 5'- gACUGCCUCAUCgaacgucaGGcaaUACGu-GGCGCg -3' miRNA: 3'- aUGAUGGAGUGGa-------CC---AUGCuuCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 48012 | 0.7 | 0.532684 |
Target: 5'- gACUACCggCACCg---GCGGgcAGGCGCu -3' miRNA: 3'- aUGAUGGa-GUGGaccaUGCU--UCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 17397 | 0.7 | 0.521798 |
Target: 5'- gGCgcgGCCgcgCGCCUGGcggGCGGugucgcAGGUGCa -3' miRNA: 3'- aUGa--UGGa--GUGGACCa--UGCU------UCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 17082 | 0.77 | 0.19405 |
Target: 5'- gACUGCUUgAUCUGGUGCccGGGCGCc -3' miRNA: 3'- aUGAUGGAgUGGACCAUGcuUCCGCG- -5' |
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24110 | 5' | -53.7 | NC_005263.2 | + | 48045 | 1.11 | 0.000802 |
Target: 5'- uUACUACCUCACCUGGUACGAAGGCGCg -3' miRNA: 3'- -AUGAUGGAGUGGACCAUGCUUCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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