Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24111 | 3' | -53.7 | NC_005263.2 | + | 19471 | 0.67 | 0.689032 |
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Target: 5'- -gCGUUCCGCAcGGCGcUCGAgcaguacggguuggaCGUGACg -3' miRNA: 3'- uaGUAAGGCGU-CUGC-AGCUg--------------GCACUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 9234 | 0.67 | 0.676763 |
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Target: 5'- -gCGUUCaGCAcGGCGaacgugcCGACCGUGACg -3' miRNA: 3'- uaGUAAGgCGU-CUGCa------GCUGGCACUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 13520 | 0.68 | 0.654328 |
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Target: 5'- -aCAcgUCCGCGaguaaucaagcGACGcUGACCGUGGCu -3' miRNA: 3'- uaGUa-AGGCGU-----------CUGCaGCUGGCACUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 25389 | 0.68 | 0.643073 |
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Target: 5'- cUCAUgcgCCGCgAGGCcUCGACC-UGGCa -3' miRNA: 3'- uAGUAa--GGCG-UCUGcAGCUGGcACUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 16011 | 0.68 | 0.643073 |
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Target: 5'- cGUCGaaaUUCCGCAGAuCGcaUCGAgCGUcGACg -3' miRNA: 3'- -UAGU---AAGGCGUCU-GC--AGCUgGCA-CUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 20477 | 0.68 | 0.643073 |
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Target: 5'- cGUCGaUCgGCcaugAGugGUCGACCGUcGCg -3' miRNA: 3'- -UAGUaAGgCG----UCugCAGCUGGCAcUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 46070 | 0.7 | 0.528342 |
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Target: 5'- ------gCGCGGGCGUCGacgugaucgaugcgGCCGUGACg -3' miRNA: 3'- uaguaagGCGUCUGCAGC--------------UGGCACUG- -5' |
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| 24111 | 3' | -53.7 | NC_005263.2 | + | 47815 | 1.07 | 0.001708 |
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Target: 5'- cAUCAUUCCGCAGACGUCGACCGUGACc -3' miRNA: 3'- -UAGUAAGGCGUCUGCAGCUGGCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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