Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24113 | 5' | -49 | NC_005263.2 | + | 17625 | 0.66 | 0.950787 |
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Target: 5'- cGCGGGUCgcgcAUCGaugcgUUCcgUCGGCg -3' miRNA: 3'- aCGCCCGGaac-UAGUa----AAGuaAGCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 16206 | 0.66 | 0.945912 |
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Target: 5'- aGCGaGGCUUUGAUCGguugcaggUCAgccauacCGGCg -3' miRNA: 3'- aCGC-CCGGAACUAGUaa------AGUaa-----GCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 788 | 0.67 | 0.910133 |
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Target: 5'- cUGCGGGUCgacGUCGgcacccagUCcgUCGACa -3' miRNA: 3'- -ACGCCCGGaacUAGUaa------AGuaAGCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 25828 | 0.68 | 0.880095 |
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Target: 5'- uUGCGGGCCguuuGUCGUUUCug-CG-Ca -3' miRNA: 3'- -ACGCCCGGaac-UAGUAAAGuaaGCuG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 4675 | 0.7 | 0.795482 |
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Target: 5'- cGCGGGUCUUucgucagGAUCGUg-CcgUCGGCg -3' miRNA: 3'- aCGCCCGGAA-------CUAGUAaaGuaAGCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 24390 | 0.7 | 0.786095 |
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Target: 5'- uUGCGGGCUggUUGGUCugugcggUCAUgUCGAUc -3' miRNA: 3'- -ACGCCCGG--AACUAGuaa----AGUA-AGCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 26345 | 0.7 | 0.764723 |
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Target: 5'- uUGCGGGaCgUUGGUCGUggUCAUgcgcgCGAUc -3' miRNA: 3'- -ACGCCC-GgAACUAGUAa-AGUAa----GCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 22203 | 0.72 | 0.685781 |
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Target: 5'- gGCGuGGCCcgGggCAUUUCGUcgCGACg -3' miRNA: 3'- aCGC-CCGGaaCuaGUAAAGUAa-GCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 1027 | 0.86 | 0.113504 |
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Target: 5'- aUGCGGGCCUUGAUCuggaUCAggcacUUCGACg -3' miRNA: 3'- -ACGCCCGGAACUAGuaa-AGU-----AAGCUG- -5' |
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| 24113 | 5' | -49 | NC_005263.2 | + | 47103 | 1.12 | 0.002241 |
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Target: 5'- uUGCGGGCCUUGAUCAUUUCAUUCGACg -3' miRNA: 3'- -ACGCCCGGAACUAGUAAAGUAAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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