Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24124 | 3' | -46.6 | NC_005263.2 | + | 41139 | 0.66 | 0.989564 |
Target: 5'- gCGAGuauccGGAAACCGGcguucgcGCGGCGUGg -3' miRNA: 3'- -GCUUcuu--CUUUUGGCCau-----UGCCGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 5522 | 0.66 | 0.989564 |
Target: 5'- gCGAGGAuauAGGucaugccguGGCCGGcAACGGCcUGg -3' miRNA: 3'- -GCUUCU---UCUu--------UUGGCCaUUGCCGuAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 19910 | 0.66 | 0.987949 |
Target: 5'- ------uGAAcGCCGGcAACGGCAUGc -3' miRNA: 3'- gcuucuuCUUuUGGCCaUUGCCGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 22187 | 0.66 | 0.987949 |
Target: 5'- ----cGGGcGAACCGGUGcUGGCGUGg -3' miRNA: 3'- gcuucUUCuUUUGGCCAUuGCCGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 27753 | 0.67 | 0.981934 |
Target: 5'- aCGAAGcuguGGAucAGGCCGGUAuCGGUg-- -3' miRNA: 3'- -GCUUCu---UCU--UUUGGCCAUuGCCGuac -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 42750 | 0.67 | 0.976824 |
Target: 5'- uCGAcccGAAGAAu-CCGGgcauccacGGCGGCGUGa -3' miRNA: 3'- -GCUu--CUUCUUuuGGCCa-------UUGCCGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 38119 | 0.67 | 0.976824 |
Target: 5'- gCGcAAGAcGGcGAACCGG--ACGGCGUGc -3' miRNA: 3'- -GC-UUCU-UCuUUUGGCCauUGCCGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 39533 | 0.69 | 0.944179 |
Target: 5'- gCGAAucGAAGAAAggcgacuggacgacGCUGGUcGCGGCAUu -3' miRNA: 3'- -GCUU--CUUCUUU--------------UGGCCAuUGCCGUAc -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 38195 | 0.7 | 0.90754 |
Target: 5'- aCGAGGAAGAAGgcccgacgccgcgcGCCGacGACGaGCAUGa -3' miRNA: 3'- -GCUUCUUCUUU--------------UGGCcaUUGC-CGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 17020 | 0.72 | 0.8444 |
Target: 5'- cCGAcuucGAGAAcgaugucgcagcGGCUGGUGGCGGCGUGa -3' miRNA: 3'- -GCUuc--UUCUU------------UUGGCCAUUGCCGUAC- -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 24679 | 0.76 | 0.624794 |
Target: 5'- aCGAucGGggGc--GCCGGUAACGGCGg- -3' miRNA: 3'- -GCU--UCuuCuuuUGGCCAUUGCCGUac -5' |
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24124 | 3' | -46.6 | NC_005263.2 | + | 42458 | 1.11 | 0.005624 |
Target: 5'- uCGAAGAAGAAAACCGGUAACGGCAUGg -3' miRNA: 3'- -GCUUCUUCUUUUGGCCAUUGCCGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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