Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24125 | 3' | -52.5 | NC_005263.2 | + | 13960 | 0.69 | 0.6244 |
Target: 5'- aGGACgcg-GCgcGCGUucgCGGGGCGUGu -3' miRNA: 3'- -UCUGaacaCGauUGCAa--GCCCCGCAU- -5' |
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24125 | 3' | -52.5 | NC_005263.2 | + | 5702 | 0.72 | 0.479828 |
Target: 5'- uGACUUGUGaaaUGAa-UUCGGGGCGg- -3' miRNA: 3'- uCUGAACACg--AUUgcAAGCCCCGCau -5' |
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24125 | 3' | -52.5 | NC_005263.2 | + | 22187 | 0.74 | 0.371389 |
Target: 5'- cGGGCgaaccgGUGCUGGCGUggccCGGGGCa-- -3' miRNA: 3'- -UCUGaa----CACGAUUGCAa---GCCCCGcau -5' |
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24125 | 3' | -52.5 | NC_005263.2 | + | 42366 | 1.07 | 0.002137 |
Target: 5'- uAGACUUGUGCUAACGUUCGGGGCGUAc -3' miRNA: 3'- -UCUGAACACGAUUGCAAGCCCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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