Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24127 | 3' | -55.7 | NC_005263.2 | + | 40692 | 0.66 | 0.651615 |
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Target: 5'- gCUCGaCGCG-CCGAUCUCCCg----- -3' miRNA: 3'- -GAGCcGCGCaGGUUAGAGGGgcuuaa -5' |
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| 24127 | 3' | -55.7 | NC_005263.2 | + | 26928 | 0.66 | 0.629347 |
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Target: 5'- -cCGcGCGCGUCCGcGUCcgCCCCGu--- -3' miRNA: 3'- gaGC-CGCGCAGGU-UAGa-GGGGCuuaa -5' |
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| 24127 | 3' | -55.7 | NC_005263.2 | + | 6327 | 0.68 | 0.536806 |
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Target: 5'- -cCGGCGCGcgagucgcccgcgCCGAUCUCCUCGu--- -3' miRNA: 3'- gaGCCGCGCa------------GGUUAGAGGGGCuuaa -5' |
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| 24127 | 3' | -55.7 | NC_005263.2 | + | 42382 | 0.68 | 0.519638 |
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Target: 5'- uUCGGgGCGUaCCcuucaaGGUCcgCCCCGAAUUc -3' miRNA: 3'- gAGCCgCGCA-GG------UUAGa-GGGGCUUAA- -5' |
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| 24127 | 3' | -55.7 | NC_005263.2 | + | 20792 | 0.69 | 0.477736 |
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Target: 5'- gUCGGCGCGUCgGGUCgcgCCgUGGu-- -3' miRNA: 3'- gAGCCGCGCAGgUUAGa--GGgGCUuaa -5' |
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| 24127 | 3' | -55.7 | NC_005263.2 | + | 41857 | 1.06 | 0.001125 |
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Target: 5'- aCUCGGCGCGUCCAAUCUCCCCGAAUUg -3' miRNA: 3'- -GAGCCGCGCAGGUUAGAGGGGCUUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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