Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24127 | 5' | -52.5 | NC_005263.2 | + | 19867 | 0.66 | 0.86525 |
Target: 5'- aGGUCacGGcGAGcgcGGCgAGCGCGGCCg -3' miRNA: 3'- cCUAGa-CC-CUCuu-CUGgUUGCGCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 2749 | 0.66 | 0.856919 |
Target: 5'- uGAUCUGcGAGcAGAagu-CGCGACCg -3' miRNA: 3'- cCUAGACcCUCuUCUgguuGCGCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 11650 | 0.66 | 0.856919 |
Target: 5'- ---cCUGcGAGAucgagcGGAUCAGCGCGACg -3' miRNA: 3'- ccuaGACcCUCU------UCUGGUUGCGCUGg -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 19195 | 0.66 | 0.839539 |
Target: 5'- ----aUGGGAc-GGAUCAGCGCGGCg -3' miRNA: 3'- ccuagACCCUcuUCUGGUUGCGCUGg -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 32424 | 0.66 | 0.836852 |
Target: 5'- aGGAUgaGGGAauggucagcggaaaGAAcGuguCCGACGCGACg -3' miRNA: 3'- -CCUAgaCCCU--------------CUU-Cu--GGUUGCGCUGg -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 9348 | 0.66 | 0.830507 |
Target: 5'- cGGAUCguaucGGuGAAGGCgAGCGcCGGCg -3' miRNA: 3'- -CCUAGac---CCuCUUCUGgUUGC-GCUGg -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 30333 | 0.67 | 0.80216 |
Target: 5'- -aAUUUGcGAGAAGcgcguGCCgAGCGCGGCCg -3' miRNA: 3'- ccUAGACcCUCUUC-----UGG-UUGCGCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 44332 | 0.67 | 0.801185 |
Target: 5'- cGGcGUgUGGGAGAAcACCGugccgcugcccgcGCGCGAgCa -3' miRNA: 3'- -CC-UAgACCCUCUUcUGGU-------------UGCGCUgG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 45894 | 0.68 | 0.772163 |
Target: 5'- uGAUCgcgcagGGGcGaAAGACCAGauguggGCGACCg -3' miRNA: 3'- cCUAGa-----CCCuC-UUCUGGUUg-----CGCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 47316 | 0.68 | 0.761851 |
Target: 5'- cGGA-CUGGGu----GCCGACGuCGACCc -3' miRNA: 3'- -CCUaGACCCucuucUGGUUGC-GCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 10914 | 0.69 | 0.686493 |
Target: 5'- cGcgCUGGGGGAu-GCCGcACGUGAUCg -3' miRNA: 3'- cCuaGACCCUCUucUGGU-UGCGCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 41765 | 0.69 | 0.686493 |
Target: 5'- cGAUCaacGAGGAGAUCGGCGCGGgCg -3' miRNA: 3'- cCUAGaccCUCUUCUGGUUGCGCUgG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 5374 | 0.7 | 0.630808 |
Target: 5'- cGGGUCgaucGGGuuGAAG-CCGGCGuUGACCu -3' miRNA: 3'- -CCUAGa---CCCu-CUUCuGGUUGC-GCUGG- -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 22150 | 0.71 | 0.597324 |
Target: 5'- gGGcgCUGuGGucGAaccAGACCGACGUGACg -3' miRNA: 3'- -CCuaGAC-CCu-CU---UCUGGUUGCGCUGg -5' |
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24127 | 5' | -52.5 | NC_005263.2 | + | 41894 | 1.03 | 0.00518 |
Target: 5'- uGGAUCUGGGAGAAGACCAACGC-ACCg -3' miRNA: 3'- -CCUAGACCCUCUUCUGGUUGCGcUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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