miRNA: 3'- caUAGGcCuu-UGGCCGcAAGCGCGCC- -5'
miRNA: 3'- -CAUAGGC--CuuUGGCCGCAagCGCGCC- -5'
miRNA: 3'- cauaGGCCUU---UG-GCCGCAAGCGC-GCC- -5'
miRNA: 3'- -CAUAGGCCUUUGGCCGCAAGCGCGCC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24128 | 5' | -55 | NC_005263.2 | + | 12503 | 0.72 | 0.362145 |
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Target: 5'- -cAUCCuGcgcGCCGGC-UUCGCGCGGg -3' miRNA: 3'- caUAGGcCuu-UGGCCGcAAGCGCGCC- -5' |
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| 24128 | 5' | -55 | NC_005263.2 | + | 12859 | 0.74 | 0.313335 |
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Target: 5'- cGUAUUCGauGggGCCGGCGU--GCGUGGu -3' miRNA: 3'- -CAUAGGC--CuuUGGCCGCAagCGCGCC- -5' |
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| 24128 | 5' | -55 | NC_005263.2 | + | 22887 | 0.75 | 0.249746 |
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Target: 5'- ---gCCGGGAucgGCaCGGCGUUCGUGcCGGa -3' miRNA: 3'- cauaGGCCUU---UG-GCCGCAAGCGC-GCC- -5' |
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| 24128 | 5' | -55 | NC_005263.2 | + | 41142 | 1.1 | 0.000988 |
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Target: 5'- aGUAUCCGGAAACCGGCGUUCGCGCGGc -3' miRNA: 3'- -CAUAGGCCUUUGGCCGCAAGCGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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