Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24129 | 3' | -56.6 | NC_005263.2 | + | 20810 | 0.66 | 0.653494 |
Target: 5'- gCCGUGGUAuUUCugcaGGCGCGGCucGGCc -3' miRNA: 3'- -GGCACCGUcAAGc---CUGUGCCGcuCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 20886 | 0.66 | 0.653494 |
Target: 5'- gCCaGUGGCAGga-GGcGCACGG-GAuGACg -3' miRNA: 3'- -GG-CACCGUCaagCC-UGUGCCgCU-CUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 9533 | 0.66 | 0.652406 |
Target: 5'- gCCGUGcGCGGcagCGaccGACGCGGCGcucgcggcuugccAGGCg -3' miRNA: 3'- -GGCAC-CGUCaa-GC---CUGUGCCGC-------------UCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 31010 | 0.66 | 0.620793 |
Target: 5'- aUCGUGcaGCAGUgcggCGaGGCGCGGCGuGcGCu -3' miRNA: 3'- -GGCAC--CGUCAa---GC-CUGUGCCGCuC-UG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 11406 | 0.66 | 0.620793 |
Target: 5'- aCGUGGU---UCGGGCGCGGCu---- -3' miRNA: 3'- gGCACCGucaAGCCUGUGCCGcucug -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 41735 | 0.66 | 0.609899 |
Target: 5'- aCGcgGGCGGcaacUUCGGgcgGCACGGCGcGAUc -3' miRNA: 3'- gGCa-CCGUC----AAGCC---UGUGCCGCuCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 43 | 0.67 | 0.566601 |
Target: 5'- gCGcGGCAGgUCGGGCGCgccuucguaccaGGUGAGGu -3' miRNA: 3'- gGCaCCGUCaAGCCUGUG------------CCGCUCUg -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 15717 | 0.67 | 0.545232 |
Target: 5'- aCG-GGCGGUUCGaccaGCGCGGCGuuaGCg -3' miRNA: 3'- gGCaCCGUCAAGCc---UGUGCCGCuc-UG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 25675 | 0.68 | 0.524129 |
Target: 5'- aCGUaGCAauccGUcacccCGGACACGGCGAGcACg -3' miRNA: 3'- gGCAcCGU----CAa----GCCUGUGCCGCUC-UG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 24924 | 0.69 | 0.472871 |
Target: 5'- ---gGGCucuaacccGUUCGGugGCGGCG-GACg -3' miRNA: 3'- ggcaCCGu-------CAAGCCugUGCCGCuCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 25018 | 0.69 | 0.46292 |
Target: 5'- gCgGUGGCGGUggcugCGGuggcguguACGCGGC-AGGCa -3' miRNA: 3'- -GgCACCGUCAa----GCC--------UGUGCCGcUCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 5572 | 0.69 | 0.447231 |
Target: 5'- aCCGUucagcucgauugugcGGCcg-UCGGgcauccGCACGGCGAGGCc -3' miRNA: 3'- -GGCA---------------CCGucaAGCC------UGUGCCGCUCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 35711 | 0.7 | 0.414903 |
Target: 5'- gCCGgccGGCAaggUCGaAgACGGCGAGACg -3' miRNA: 3'- -GGCa--CCGUca-AGCcUgUGCCGCUCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 27534 | 0.7 | 0.405672 |
Target: 5'- cCCGUcggcacggccGGCAGgcCGGGCacgagcgucGCGGCGAGGu -3' miRNA: 3'- -GGCA----------CCGUCaaGCCUG---------UGCCGCUCUg -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 31999 | 0.7 | 0.405672 |
Target: 5'- aCUGUGGCGGgagUGGcACuguggGCGGCGAGcGCa -3' miRNA: 3'- -GGCACCGUCaa-GCC-UG-----UGCCGCUC-UG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 22504 | 0.71 | 0.361508 |
Target: 5'- gCCGgcGGCGGUUCGGcgGCuGCGGCcugcucgaugccGAGACg -3' miRNA: 3'- -GGCa-CCGUCAAGCC--UG-UGCCG------------CUCUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 14243 | 0.71 | 0.353086 |
Target: 5'- cCCGUGGCGug-CGGGCuggcuuGCGGCGGccGGCg -3' miRNA: 3'- -GGCACCGUcaaGCCUG------UGCCGCU--CUG- -5' |
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24129 | 3' | -56.6 | NC_005263.2 | + | 40779 | 1.12 | 0.000462 |
Target: 5'- gCCGUGGCAGUUCGGACACGGCGAGACg -3' miRNA: 3'- -GGCACCGUCAAGCCUGUGCCGCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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