Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24129 | 5' | -53.6 | NC_005263.2 | + | 29872 | 0.66 | 0.763972 |
Target: 5'- uUGCUUGUGGCguUCGucuugaUGCCGUg -3' miRNA: 3'- gACGGACACCG--AGUuucuugACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 37983 | 0.66 | 0.762918 |
Target: 5'- -cGCUgaagGUGGCggCAAcGAACUcgccacgauugguGCCGCa -3' miRNA: 3'- gaCGGa---CACCGa-GUUuCUUGA-------------CGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 35891 | 0.66 | 0.753373 |
Target: 5'- -cGCauucgacUGGUUCGAuccggacGAGCUGCCGCa -3' miRNA: 3'- gaCGgac----ACCGAGUUu------CUUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 44153 | 0.66 | 0.720839 |
Target: 5'- -gGCCUGccaGGCUCGcu-GGCUGgCGCg -3' miRNA: 3'- gaCGGACa--CCGAGUuucUUGACgGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 38489 | 0.66 | 0.709789 |
Target: 5'- uUGUCgauccGUGGCgcgacgCGAuGAucgcGCUGCCGCa -3' miRNA: 3'- gACGGa----CACCGa-----GUUuCU----UGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 3396 | 0.67 | 0.698658 |
Target: 5'- -cGCCgucguugcgGGCacuucggUAAAGAACUGCUGCg -3' miRNA: 3'- gaCGGaca------CCGa------GUUUCUUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 36799 | 0.68 | 0.63085 |
Target: 5'- uUGCCUGgcgaGGC-CAua-AAUUGCCGCu -3' miRNA: 3'- gACGGACa---CCGaGUuucUUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 34011 | 0.68 | 0.63085 |
Target: 5'- gCUGCgUcGcGGCgcgCGAuccAGAGCUGCUGCg -3' miRNA: 3'- -GACGgA-CaCCGa--GUU---UCUUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 46606 | 0.68 | 0.608136 |
Target: 5'- -cGCC-GUGcGCUCgAAAGAAaccgagUGCCGCg -3' miRNA: 3'- gaCGGaCAC-CGAG-UUUCUUg-----ACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 3170 | 0.68 | 0.596806 |
Target: 5'- -gGCUUcGUGGCgcgGAAuAACUGCCGCu -3' miRNA: 3'- gaCGGA-CACCGag-UUUcUUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 17640 | 0.68 | 0.596806 |
Target: 5'- -cGCCggaUGUGGCgcaacgCGAAG-GCcGCCGCg -3' miRNA: 3'- gaCGG---ACACCGa-----GUUUCuUGaCGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 12207 | 0.68 | 0.594544 |
Target: 5'- -cGCCUGUGuCUCGaccgcaccgacaucaAcgcggcgaaguauuuGGGGCUGCCGCa -3' miRNA: 3'- gaCGGACACcGAGU---------------U---------------UCUUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 31267 | 0.69 | 0.551903 |
Target: 5'- -cGCCg--GGCaggUCGAAGuGCUGUCGCa -3' miRNA: 3'- gaCGGacaCCG---AGUUUCuUGACGGCG- -5' |
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24129 | 5' | -53.6 | NC_005263.2 | + | 40814 | 1.11 | 0.000745 |
Target: 5'- cCUGCCUGUGGCUCAAAGAACUGCCGCc -3' miRNA: 3'- -GACGGACACCGAGUUUCUUGACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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