Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24136 | 5' | -51.4 | NC_005263.2 | + | 21930 | 0.7 | 0.634121 |
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Target: 5'- cGCGGCCGCag-ACCguccucauccACCGGACAAc -3' miRNA: 3'- -CGUCGGCGcuaUGGaau-------UGGUCUGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 18463 | 0.7 | 0.613277 |
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Target: 5'- aGCAGCCGCGcgGCUgguacacccuUUGGCUgcaGGGCGAa -3' miRNA: 3'- -CGUCGGCGCuaUGG----------AAUUGG---UCUGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 45364 | 0.71 | 0.590188 |
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Target: 5'- gGCGGCCGCGAUcucgGCUgUGACgGGgACGAa -3' miRNA: 3'- -CGUCGGCGCUA----UGGaAUUGgUC-UGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 16500 | 0.71 | 0.590188 |
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Target: 5'- cGCGGCCGuCGcgACCggcaUGACCAaccGGCAAa -3' miRNA: 3'- -CGUCGGC-GCuaUGGa---AUUGGU---CUGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 16619 | 0.71 | 0.567255 |
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Target: 5'- aGCAGCuCGCGccGCUgacgGGCCAGGCGc -3' miRNA: 3'- -CGUCG-GCGCuaUGGaa--UUGGUCUGUu -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 34793 | 0.73 | 0.467883 |
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Target: 5'- cGCAGCuCGCGAUGCCgu-ACaucGACAAc -3' miRNA: 3'- -CGUCG-GCGCUAUGGaauUGgu-CUGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 32847 | 0.73 | 0.467883 |
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Target: 5'- aGC-GCCGCGAcaaGCCgcagcaugUAGCCGGACAGc -3' miRNA: 3'- -CGuCGGCGCUa--UGGa-------AUUGGUCUGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 25393 | 0.74 | 0.387615 |
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Target: 5'- uGC-GCCGCGAgGCCUcGACCuGGCAGa -3' miRNA: 3'- -CGuCGGCGCUaUGGAaUUGGuCUGUU- -5' |
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| 24136 | 5' | -51.4 | NC_005263.2 | + | 38704 | 1.09 | 0.001692 |
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Target: 5'- cGCAGCCGCGAUACCUUAACCAGACAAg -3' miRNA: 3'- -CGUCGGCGCUAUGGAAUUGGUCUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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