Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24143 | 5' | -62.6 | NC_005263.2 | + | 6617 | 0.66 | 0.32124 |
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Target: 5'- cGGcg-GCGCGCUCGGCgcGGUCGAGc- -3' miRNA: 3'- aCCcagCGUGCGGGCCG--CCAGCUCuu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 24307 | 0.67 | 0.284895 |
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Target: 5'- uUGGcUCGCGCGCgucgcaUCGGCGGUCGuaacGGGc -3' miRNA: 3'- -ACCcAGCGUGCG------GGCCGCCAGC----UCUu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 35704 | 0.67 | 0.271293 |
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Target: 5'- gGGGUCuGC-CGgCCGGCaaGGUCGaAGAc -3' miRNA: 3'- aCCCAG-CGuGCgGGCCG--CCAGC-UCUu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 35829 | 0.67 | 0.251876 |
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Target: 5'- cGGGUCGCuugUGCCCuGCaGGUCGGc-- -3' miRNA: 3'- aCCCAGCGu--GCGGGcCG-CCAGCUcuu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 39891 | 0.68 | 0.239577 |
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Target: 5'- gGGGUCGC-CGgCUGucgaaGCGGUCGuGAAg -3' miRNA: 3'- aCCCAGCGuGCgGGC-----CGCCAGCuCUU- -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 12118 | 0.69 | 0.181136 |
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Target: 5'- cGGcG-CGCGCGCCauuacgcgcgcuccaGGCGGUCGAGc- -3' miRNA: 3'- aCC-CaGCGUGCGGg--------------CCGCCAGCUCuu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 17399 | 0.7 | 0.166984 |
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Target: 5'- cGcGGcCGCGCGCCUGGCGGgCGGu-- -3' miRNA: 3'- aC-CCaGCGUGCGGGCCGCCaGCUcuu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 26416 | 0.72 | 0.121145 |
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Target: 5'- aGGGUCagggGCGCGUCaGGCGGUCGAu-- -3' miRNA: 3'- aCCCAG----CGUGCGGgCCGCCAGCUcuu -5' |
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| 24143 | 5' | -62.6 | NC_005263.2 | + | 36853 | 1.05 | 0.000298 |
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Target: 5'- aUGGGUCGCACGCCCGGCGGUCGAGAAu -3' miRNA: 3'- -ACCCAGCGUGCGGGCCGCCAGCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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