Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24145 | 5' | -52.5 | NC_005263.2 | + | 17551 | 0.66 | 0.839273 |
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Target: 5'- uGAAGucguUGUGGGAGGACGUCGaGCa- -3' miRNA: 3'- gCUUCc---ACGUCCUUUUGCAGCgCGac -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 11779 | 0.66 | 0.811128 |
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Target: 5'- gGgcGGUGCAuaucgcauGGcgcAGGACGUCGCGUg- -3' miRNA: 3'- gCuuCCACGU--------CC---UUUUGCAGCGCGac -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 22537 | 0.67 | 0.760395 |
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Target: 5'- uGAcGGgcgGCGcGGAcgccGACGUCGCGCUc -3' miRNA: 3'- gCUuCCa--CGU-CCUu---UUGCAGCGCGAc -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 43141 | 0.68 | 0.728188 |
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Target: 5'- uGGAGcGcGCcGGAAcucaguCGUCGCGCUGg -3' miRNA: 3'- gCUUC-CaCGuCCUUuu----GCAGCGCGAC- -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 10847 | 0.7 | 0.571003 |
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Target: 5'- uCGuuGGUGCGGGcuacGGCGUCGCGg-- -3' miRNA: 3'- -GCuuCCACGUCCuu--UUGCAGCGCgac -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 41203 | 0.71 | 0.537727 |
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Target: 5'- gCGAAGGUGUAcGAucuGACGUCGgGCc- -3' miRNA: 3'- -GCUUCCACGUcCUu--UUGCAGCgCGac -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 16409 | 0.71 | 0.515916 |
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Target: 5'- uCGcGGGUGCGGuacucGGCGUCGgCGCUGg -3' miRNA: 3'- -GCuUCCACGUCcuu--UUGCAGC-GCGAC- -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 2665 | 0.71 | 0.515916 |
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Target: 5'- -cGAGGUGCAGGcguGCGUUGUgguaGCUGa -3' miRNA: 3'- gcUUCCACGUCCuuuUGCAGCG----CGAC- -5' |
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| 24145 | 5' | -52.5 | NC_005263.2 | + | 36436 | 1.09 | 0.00153 |
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Target: 5'- gCGAAGGUGCAGGAAAACGUCGCGCUGa -3' miRNA: 3'- -GCUUCCACGUCCUUUUGCAGCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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