Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24146 | 5' | -53.2 | NC_005263.2 | + | 3706 | 0.66 | 0.746252 |
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Target: 5'- gUUCGaAUGGuuUGAuugAGCGCCUcGCGCa- -3' miRNA: 3'- -AAGC-UGCUuuACU---UCGCGGA-CGCGcu -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 20178 | 0.66 | 0.746252 |
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Target: 5'- aUCGACGAc--GAGcGCGCC-GgGCGAc -3' miRNA: 3'- aAGCUGCUuuaCUU-CGCGGaCgCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 1053 | 0.66 | 0.756971 |
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Target: 5'- cUUCGACGAAuc--AGCGCUUGUaauccuuaGCGAc -3' miRNA: 3'- -AAGCUGCUUuacuUCGCGGACG--------CGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 41526 | 0.66 | 0.765448 |
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Target: 5'- gUCGACuacuu---GCGCCUGCGCa- -3' miRNA: 3'- aAGCUGcuuuacuuCGCGGACGCGcu -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 15469 | 0.66 | 0.767552 |
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Target: 5'- -gCGACGAuuUGccGUGCUgcggcgGCGCGGu -3' miRNA: 3'- aaGCUGCUuuACuuCGCGGa-----CGCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 38089 | 0.66 | 0.777985 |
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Target: 5'- cUCGGCGGcGGUGcuGCGCCcgcaccugGCGCGc -3' miRNA: 3'- aAGCUGCU-UUACuuCGCGGa-------CGCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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