Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24146 | 5' | -53.2 | NC_005263.2 | + | 9452 | 0.71 | 0.48955 |
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Target: 5'- cUUGGCGAGGUu-GGCGUCgaUGCGCGAc -3' miRNA: 3'- aAGCUGCUUUAcuUCGCGG--ACGCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 7274 | 0.68 | 0.642304 |
|
Target: 5'- aUCGACGggGcgagcgacaUGAcGCGUgccugcggccagguCUGCGCGAu -3' miRNA: 3'- aAGCUGCuuU---------ACUuCGCG--------------GACGCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 3706 | 0.66 | 0.746252 |
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Target: 5'- gUUCGaAUGGuuUGAuugAGCGCCUcGCGCa- -3' miRNA: 3'- -AAGC-UGCUuuACU---UCGCGGA-CGCGcu -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 1060 | 0.68 | 0.650277 |
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Target: 5'- -gCGGCGcuugcgaggaggcuuGAcgGAAGUGCCgaauUGCGCGAc -3' miRNA: 3'- aaGCUGC---------------UUuaCUUCGCGG----ACGCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 1053 | 0.66 | 0.756971 |
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Target: 5'- cUUCGACGAAuc--AGCGCUUGUaauccuuaGCGAc -3' miRNA: 3'- -AAGCUGCUUuacuUCGCGGACG--------CGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 961 | 0.7 | 0.532891 |
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Target: 5'- -gCGACGuAGcgGcGGuCGCCUGCGCGu -3' miRNA: 3'- aaGCUGC-UUuaCuUC-GCGGACGCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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