Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24146 | 5' | -53.2 | NC_005263.2 | + | 11890 | 0.72 | 0.427864 |
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Target: 5'- -aCGACGcggcgccGGGCGCCUGCGCGc -3' miRNA: 3'- aaGCUGCuuuac--UUCGCGGACGCGCu -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 32887 | 0.71 | 0.478969 |
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Target: 5'- -gCGGCGAAAUuucguugacccgGAAGCGCUUGC-CGAc -3' miRNA: 3'- aaGCUGCUUUA------------CUUCGCGGACGcGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 9452 | 0.71 | 0.48955 |
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Target: 5'- cUUGGCGAGGUu-GGCGUCgaUGCGCGAc -3' miRNA: 3'- aAGCUGCUUUAcuUCGCGG--ACGCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 21376 | 0.7 | 0.521916 |
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Target: 5'- cUCGACGuGAUGguGCGCUUcGCGCu- -3' miRNA: 3'- aAGCUGCuUUACuuCGCGGA-CGCGcu -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 27093 | 0.7 | 0.521916 |
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Target: 5'- aUCGAUGAAGUcuGGCGCUucgacggGCGCGGc -3' miRNA: 3'- aAGCUGCUUUAcuUCGCGGa------CGCGCU- -5' |
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| 24146 | 5' | -53.2 | NC_005263.2 | + | 15469 | 0.66 | 0.767552 |
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Target: 5'- -gCGACGAuuUGccGUGCUgcggcgGCGCGGu -3' miRNA: 3'- aaGCUGCUuuACuuCGCGGa-----CGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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