Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24151 | 3' | -54.8 | NC_005263.2 | + | 13423 | 0.66 | 0.723779 |
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Target: 5'- -gCCGGcgccuugaUCCCGGGCUcagcaaaUUCCacCGCa -3' miRNA: 3'- caGGCCaa------AGGGCCCGA-------AAGGauGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 10177 | 0.66 | 0.692322 |
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Target: 5'- -aCCGGcugCCgGcGGCgcgCCUGCGCc -3' miRNA: 3'- caGGCCaaaGGgC-CCGaaaGGAUGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 11895 | 0.66 | 0.692322 |
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Target: 5'- --gCGGcg--CCGGGCg--CCUGCGCg -3' miRNA: 3'- cagGCCaaagGGCCCGaaaGGAUGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 10210 | 0.66 | 0.681345 |
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Target: 5'- -gCCGGc--CUCGGGCUcgagUCCagACGCg -3' miRNA: 3'- caGGCCaaaGGGCCCGAa---AGGa-UGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 29553 | 0.67 | 0.659259 |
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Target: 5'- -aCCGGUg--CCGGGCUgcgcgaacUUCUUgaACGCg -3' miRNA: 3'- caGGCCAaagGGCCCGA--------AAGGA--UGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 25932 | 0.67 | 0.625964 |
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Target: 5'- -aCCGGUUcgCCCGGcGCUg----GCGCa -3' miRNA: 3'- caGGCCAAa-GGGCC-CGAaaggaUGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 25518 | 0.68 | 0.559773 |
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Target: 5'- -gUCGGcgUCUCGGGCUggCUUgGCGCa -3' miRNA: 3'- caGGCCaaAGGGCCCGAaaGGA-UGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 17091 | 0.7 | 0.444902 |
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Target: 5'- aUCUGGUg-CCCGGGCg--CCcGCGUg -3' miRNA: 3'- cAGGCCAaaGGGCCCGaaaGGaUGCG- -5' |
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| 24151 | 3' | -54.8 | NC_005263.2 | + | 34223 | 1.12 | 0.00064 |
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Target: 5'- cGUCCGGUUUCCCGGGCUUUCCUACGCu -3' miRNA: 3'- -CAGGCCAAAGGGCCCGAAAGGAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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