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miRNA: 3'- cUUGACGa-GCu--CGCGCUacAGCUGCU- -5'
miRNA: 3'- -CUUGA-CGAGCuCGCGCuACAGCU-GCU- -5'
miRNA: 3'- -CUUGACGAGCuCG-CGCuaCAGcUGCU- -5'
miRNA: 3'- cuUGACGAGCuCGCGCUacaGCUGCU- -5'
miRNA: 3'- -CUUGACGAGCUCGCGCUACAGCUGCU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 24164 | 3' | -53.8 | NC_005263.2 | + | 37772 | 0.71 | 0.48322 |
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Target: 5'- aAGCUGCcgCGccuGCGCGAgaUCGACGAg -3' miRNA: 3'- cUUGACGa-GCu--CGCGCUacAGCUGCU- -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 45000 | 0.73 | 0.367499 |
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Target: 5'- aGAACUgGUUCG-GCGCGcUGUCGAaCGAg -3' miRNA: 3'- -CUUGA-CGAGCuCGCGCuACAGCU-GCU- -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 17585 | 0.74 | 0.325227 |
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Target: 5'- cGAGCUGCUCGcGCaGCGggGUC-ACGAc -3' miRNA: 3'- -CUUGACGAGCuCG-CGCuaCAGcUGCU- -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 31509 | 0.76 | 0.232627 |
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Target: 5'- -uGCUGCUCGcGCGCGAgccCGGCGAa -3' miRNA: 3'- cuUGACGAGCuCGCGCUacaGCUGCU- -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 28495 | 1.09 | 0.001245 |
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Target: 5'- uGAACUGCUCGAGCGCGAUGUCGACGAa -3' miRNA: 3'- -CUUGACGAGCUCGCGCUACAGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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