miRNA: 3'- cuUGACGAGCUcGCGCUa---CAGCUGcu -5'
miRNA: 3'- cuUGACGAGCuCGCGCUaCaGCUGCu -5'
miRNA: 3'- cUUG-ACGAGC--UCGCGCUaCAg----CUGCU- -5'
miRNA: 3'- -CUUGACG---AGCU-CGCG-------------CUaCAGCUGCU- -5'
miRNA: 3'- -CUUGA-CGAGCuCGCGCuACAGCU-GCU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24164 | 3' | -53.8 | NC_005263.2 | + | 41477 | 0.7 | 0.547275 |
|
Target: 5'- -cGCUGCUCGA-CGCGGgcgaGUCGGCc- -3' miRNA: 3'- cuUGACGAGCUcGCGCUa---CAGCUGcu -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 43043 | 0.66 | 0.756678 |
|
Target: 5'- -cGCUGUUUG-GCGCGAcGcCGACGu -3' miRNA: 3'- cuUGACGAGCuCGCGCUaCaGCUGCu -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 43376 | 0.66 | 0.756678 |
|
Target: 5'- uGGCgUGCUCGcgGGCGCGAcGUacccgGACGAg -3' miRNA: 3'- cUUG-ACGAGC--UCGCGCUaCAg----CUGCU- -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 44650 | 0.65 | 0.786445 |
|
Target: 5'- cGAGCUGCgccUCGAuGCGUcgcgcgcgccgaaGAaGUCGGCGGc -3' miRNA: 3'- -CUUGACG---AGCU-CGCG-------------CUaCAGCUGCU- -5' |
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| 24164 | 3' | -53.8 | NC_005263.2 | + | 45000 | 0.73 | 0.367499 |
|
Target: 5'- aGAACUgGUUCG-GCGCGcUGUCGAaCGAg -3' miRNA: 3'- -CUUGA-CGAGCuCGCGCuACAGCU-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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