Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24184 | 3' | -50.9 | NC_005263.2 | + | 26040 | 0.66 | 0.884723 |
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Target: 5'- -gGCGGUUGAgCuCGUCGAgcuUCGC-UGCg -3' miRNA: 3'- aaCGCUAGCU-GuGCAGCU---AGCGaAUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 9766 | 0.66 | 0.884723 |
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Target: 5'- -cGcCGGUCGcCGCGUgcaCGGUCGCgcGCg -3' miRNA: 3'- aaC-GCUAGCuGUGCA---GCUAGCGaaUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 46091 | 0.66 | 0.876781 |
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Target: 5'- aUGCGGcCGugACGUCGGguaucauccgCGCcgGCg -3' miRNA: 3'- aACGCUaGCugUGCAGCUa---------GCGaaUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 19620 | 0.66 | 0.876781 |
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Target: 5'- -aGCGAacgccUCGuACGCGUUGAUgGCUg-- -3' miRNA: 3'- aaCGCU-----AGC-UGUGCAGCUAgCGAaug -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 6257 | 0.66 | 0.876781 |
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Target: 5'- -cGCGGUCGuGC-CGUCGGgcaGCUUGa -3' miRNA: 3'- aaCGCUAGC-UGuGCAGCUag-CGAAUg -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 15658 | 0.66 | 0.860072 |
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Target: 5'- -cGCGAUCGuCGCGUCGGacacguucuuUcCGCUgACc -3' miRNA: 3'- aaCGCUAGCuGUGCAGCU----------A-GCGAaUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 33810 | 0.66 | 0.899753 |
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Target: 5'- -aGCGucgaCGGCAUGaCGAUCGCgcUGCa -3' miRNA: 3'- aaCGCua--GCUGUGCaGCUAGCGa-AUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 28248 | 0.66 | 0.897572 |
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Target: 5'- -cGCGcUCGccgugaccuuguucGCAuCGUCGAUCGCgcGCg -3' miRNA: 3'- aaCGCuAGC--------------UGU-GCAGCUAGCGaaUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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