Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24184 | 3' | -50.9 | NC_005263.2 | + | 29589 | 0.71 | 0.604353 |
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Target: 5'- -cGCGGugUCGACGcCGcCGAUCGCgagGCg -3' miRNA: 3'- aaCGCU--AGCUGU-GCaGCUAGCGaa-UG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 2639 | 0.71 | 0.615858 |
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Target: 5'- -cGCGAUCGGCACGUUcg-CGCcggGCu -3' miRNA: 3'- aaCGCUAGCUGUGCAGcuaGCGaa-UG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 5459 | 0.72 | 0.581434 |
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Target: 5'- -aGCGAUCGGuugcCACGUCGG-CGCUg-- -3' miRNA: 3'- aaCGCUAGCU----GUGCAGCUaGCGAaug -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 971 | 0.7 | 0.650419 |
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Target: 5'- -gGCGGUCGccuGCGCGUCGGacggCGCUUc- -3' miRNA: 3'- aaCGCUAGC---UGUGCAGCUa---GCGAAug -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 41173 | 0.69 | 0.718709 |
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Target: 5'- -gGCGGccgucgUCGAUACGUUGcgCGCUgUGCg -3' miRNA: 3'- aaCGCU------AGCUGUGCAGCuaGCGA-AUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 43252 | 0.69 | 0.729837 |
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Target: 5'- -aGCGGcaUCGGCACGgCGcgCGCgUACg -3' miRNA: 3'- aaCGCU--AGCUGUGCaGCuaGCGaAUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 32890 | 0.68 | 0.762525 |
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Target: 5'- -gGCGAguuUCGugGCGUCGG-CGUcgGCg -3' miRNA: 3'- aaCGCU---AGCugUGCAGCUaGCGaaUG- -5' |
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| 24184 | 3' | -50.9 | NC_005263.2 | + | 33810 | 0.66 | 0.899753 |
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Target: 5'- -aGCGucgaCGGCAUGaCGAUCGCgcUGCa -3' miRNA: 3'- aaCGCua--GCUGUGCaGCUAGCGa-AUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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