Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24199 | 3' | -47.6 | NC_005263.2 | + | 403 | 0.66 | 0.96826 |
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Target: 5'- cGCgUAGAgCGCAcGAuCCuUGAUCAAGAc -3' miRNA: 3'- -UG-AUCUgGUGUuCU-GGuACUAGUUCU- -5' |
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| 24199 | 3' | -47.6 | NC_005263.2 | + | 19166 | 0.67 | 0.96065 |
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Target: 5'- ---cGACCGCAuGGGCCGUGG-CGAGc -3' miRNA: 3'- ugauCUGGUGU-UCUGGUACUaGUUCu -5' |
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| 24199 | 3' | -47.6 | NC_005263.2 | + | 21722 | 0.68 | 0.941851 |
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Target: 5'- gGCUGGaAUC-CAAGGCUcgGGUCGAGc -3' miRNA: 3'- -UGAUC-UGGuGUUCUGGuaCUAGUUCu -5' |
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| 24199 | 3' | -47.6 | NC_005263.2 | + | 482 | 0.69 | 0.889135 |
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Target: 5'- --aAGGCCGcCAGGACgAUGAgCAGGAu -3' miRNA: 3'- ugaUCUGGU-GUUCUGgUACUaGUUCU- -5' |
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| 24199 | 3' | -47.6 | NC_005263.2 | + | 32256 | 0.7 | 0.86428 |
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Target: 5'- cUUGGuuuCgACAAGGCCGUGAUCGAa- -3' miRNA: 3'- uGAUCu--GgUGUUCUGGUACUAGUUcu -5' |
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| 24199 | 3' | -47.6 | NC_005263.2 | + | 39870 | 0.73 | 0.708547 |
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Target: 5'- cACUGcGACCAC---GCCGUGGUCGGGGu -3' miRNA: 3'- -UGAU-CUGGUGuucUGGUACUAGUUCU- -5' |
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| 24199 | 3' | -47.6 | NC_005263.2 | + | 14056 | 1.1 | 0.004569 |
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Target: 5'- aACUAGACCACAAGACCAUGAUCAAGAc -3' miRNA: 3'- -UGAUCUGGUGUUCUGGUACUAGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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