Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
242 | 3' | -55.9 | AC_000008.1 | + | 5477 | 0.71 | 0.297849 |
Target: 5'- gGCGUGgcccUUgGCGCGCAGCuugcccuuggaggagGCGCCGCaCGa -3' miRNA: 3'- -UGCAU----AAgCGCGCGUUG---------------CGUGGCG-GU- -5' |
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242 | 3' | -55.9 | AC_000008.1 | + | 12741 | 0.72 | 0.245357 |
Target: 5'- uGCugcugCGCGCGCucACGCugCGCCAc -3' miRNA: 3'- -UGcauaaGCGCGCGu-UGCGugGCGGU- -5' |
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242 | 3' | -55.9 | AC_000008.1 | + | 17776 | 0.72 | 0.238883 |
Target: 5'- gGCGgcggCGCGCGU--CGCACCGUCGc -3' miRNA: 3'- -UGCauaaGCGCGCGuuGCGUGGCGGU- -5' |
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242 | 3' | -55.9 | AC_000008.1 | + | 12260 | 0.75 | 0.153567 |
Target: 5'- gACGUugccagCGCGCGCGgguGC-CACCGCCAg -3' miRNA: 3'- -UGCAuaa---GCGCGCGU---UGcGUGGCGGU- -5' |
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242 | 3' | -55.9 | AC_000008.1 | + | 6346 | 1.08 | 0.000575 |
Target: 5'- cACGUAUUCGCGCGCAACGCACCGCCAu -3' miRNA: 3'- -UGCAUAAGCGCGCGUUGCGUGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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