Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 242 | 5' | -60.9 | AC_000008.1 | + | 17655 | 0.66 | 0.307583 |
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Target: 5'- --gGGUGGUaGCGCgcuGUUGGcaGCACCAg -3' miRNA: 3'- cugCCACCA-CGCGag-CAGCC--CGUGGU- -5' |
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| 242 | 5' | -60.9 | AC_000008.1 | + | 26889 | 0.66 | 0.292722 |
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Target: 5'- cGCGGgucgauacGgGUUCGUUGGGCGCCAg -3' miRNA: 3'- cUGCCacca----CgCGAGCAGCCCGUGGU- -5' |
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| 242 | 5' | -60.9 | AC_000008.1 | + | 15724 | 0.7 | 0.173868 |
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Target: 5'- uGACGGUGGUGaugguggggGCUgG-CGGGCgcGCCAa -3' miRNA: 3'- -CUGCCACCACg--------CGAgCaGCCCG--UGGU- -5' |
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| 242 | 5' | -60.9 | AC_000008.1 | + | 5333 | 0.71 | 0.14366 |
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Target: 5'- cACGGUgaaggGGUGCGCUC--CGGGCugCGc -3' miRNA: 3'- cUGCCA-----CCACGCGAGcaGCCCGugGU- -5' |
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| 242 | 5' | -60.9 | AC_000008.1 | + | 16068 | 0.71 | 0.14366 |
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Target: 5'- cGACGGUGGUGCGCccaGUgCGGccgcguuugugcGCGCCc -3' miRNA: 3'- -CUGCCACCACGCGag-CA-GCC------------CGUGGu -5' |
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| 242 | 5' | -60.9 | AC_000008.1 | + | 6381 | 1.08 | 0.000192 |
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Target: 5'- aGACGGUGGUGCGCUCGUCGGGCACCAg -3' miRNA: 3'- -CUGCCACCACGCGAGCAGCCCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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