Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24204 | 5' | -58 | NC_005263.2 | + | 3905 | 0.68 | 0.387601 |
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Target: 5'- uUCGCacccucgGUGUCUUu-CUGCACCGGCa -3' miRNA: 3'- cAGCGga-----CACAGAGcuGGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 22781 | 0.68 | 0.386712 |
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Target: 5'- cGUCGCCgcguuUGUGagcggcuUCUCGACCGa--CGACg -3' miRNA: 3'- -CAGCGG-----ACAC-------AGAGCUGGCgugGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 41479 | 0.69 | 0.344804 |
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Target: 5'- -aCGCgCgacUG-CUCGACCGCGCCGAg -3' miRNA: 3'- caGCG-Gac-ACaGAGCUGGCGUGGCUg -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 45401 | 0.7 | 0.335051 |
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Target: 5'- cUCGCCgacgaacucgGUCugUCGGCCGCcGCCGGCg -3' miRNA: 3'- cAGCGGaca-------CAG--AGCUGGCG-UGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 9666 | 0.7 | 0.298073 |
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Target: 5'- aUCGCC---GUCUUGACCGUgGCCGGCg -3' miRNA: 3'- cAGCGGacaCAGAGCUGGCG-UGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 30951 | 0.72 | 0.249942 |
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Target: 5'- uUCGgCU---UCUCGGCCGUGCCGACg -3' miRNA: 3'- cAGCgGAcacAGAGCUGGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 27685 | 0.72 | 0.231359 |
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Target: 5'- -cCGCCU---UCUUGugCGCGCCGGCg -3' miRNA: 3'- caGCGGAcacAGAGCugGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 12204 | 1.09 | 0.000436 |
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Target: 5'- gGUCGCCUGUGUCUCGACCGCACCGACa -3' miRNA: 3'- -CAGCGGACACAGAGCUGGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 31062 | 0.66 | 0.545232 |
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Target: 5'- aGUCGCCcGUcgaucGUCUUGGCCGCGaCGcGCu -3' miRNA: 3'- -CAGCGGaCA-----CAGAGCUGGCGUgGC-UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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