Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24204 | 5' | -58 | NC_005263.2 | + | 9738 | 0.7 | 0.305508 |
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Target: 5'- uUCGCCacagcaGUGUCgaucgCgGugCGCACCGACu -3' miRNA: 3'- cAGCGGa-----CACAGa----G-CugGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 9666 | 0.7 | 0.298073 |
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Target: 5'- aUCGCC---GUCUUGACCGUgGCCGGCg -3' miRNA: 3'- cAGCGGacaCAGAGCUGGCG-UGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 8382 | 0.71 | 0.276613 |
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Target: 5'- cUCGCCaG-GUCgacgCGGcCCGCGCCGGCc -3' miRNA: 3'- cAGCGGaCaCAGa---GCU-GGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 30951 | 0.72 | 0.249942 |
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Target: 5'- uUCGgCU---UCUCGGCCGUGCCGACg -3' miRNA: 3'- cAGCgGAcacAGAGCUGGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 27685 | 0.72 | 0.231359 |
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Target: 5'- -cCGCCU---UCUUGugCGCGCCGGCg -3' miRNA: 3'- caGCGGAcacAGAGCugGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 44753 | 0.72 | 0.219628 |
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Target: 5'- -cUGUCUGgucgCUCGACCGCGCgCGGCg -3' miRNA: 3'- caGCGGACaca-GAGCUGGCGUG-GCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 17676 | 0.75 | 0.151049 |
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Target: 5'- -aCGCUgcucgcgCUCGACCGCACCGACc -3' miRNA: 3'- caGCGGacaca--GAGCUGGCGUGGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 9694 | 0.75 | 0.139137 |
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Target: 5'- cGUCGCgCUuUGccugCUCGACCGCugCGGCg -3' miRNA: 3'- -CAGCG-GAcACa---GAGCUGGCGugGCUG- -5' |
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| 24204 | 5' | -58 | NC_005263.2 | + | 12204 | 1.09 | 0.000436 |
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Target: 5'- gGUCGCCUGUGUCUCGACCGCACCGACa -3' miRNA: 3'- -CAGCGGACACAGAGCUGGCGUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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