Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24212 | 3' | -55.8 | NC_005263.2 | + | 16411 | 0.66 | 0.652376 |
Target: 5'- gCGGGUG-CGGuacuCGGcGUCGGCGCugGCg- -3' miRNA: 3'- -GUCCAUaGCUu---GCC-CAGCCGCG--UGac -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 27726 | 0.66 | 0.630299 |
Target: 5'- -uGGcgaGUUGuACGGuGUCGGCGCGCg- -3' miRNA: 3'- guCCa--UAGCuUGCC-CAGCCGCGUGac -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 44991 | 0.67 | 0.597218 |
Target: 5'- -cGGacGUCGAgaACuGGuUCGGCGCGCUGu -3' miRNA: 3'- guCCa-UAGCU--UGcCC-AGCCGCGUGAC- -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 12853 | 0.67 | 0.575298 |
Target: 5'- gCAGcccGUAuUCGAugGGGcCGGCGUGCg- -3' miRNA: 3'- -GUC---CAU-AGCUugCCCaGCCGCGUGac -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 40935 | 0.68 | 0.532084 |
Target: 5'- cCGGGUAUCGcAGCGGcGaUGGcCGCGCa- -3' miRNA: 3'- -GUCCAUAGC-UUGCC-CaGCC-GCGUGac -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 27361 | 0.69 | 0.490095 |
Target: 5'- aCAGGccgccagCGAGCGGcgcggcggcgGcCGGCGCGCUGa -3' miRNA: 3'- -GUCCaua----GCUUGCC----------CaGCCGCGUGAC- -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 4478 | 0.7 | 0.439908 |
Target: 5'- --cGUG-CGcGCGGGUCGGCGUGCUu -3' miRNA: 3'- gucCAUaGCuUGCCCAGCCGCGUGAc -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 916 | 0.7 | 0.401018 |
Target: 5'- gCGGGUuacucgucgacgcAUCGAACGGGUUGGCaccCGCg- -3' miRNA: 3'- -GUCCA-------------UAGCUUGCCCAGCCGc--GUGac -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 27164 | 0.73 | 0.272389 |
Target: 5'- -uGGUGUCguuGAACGuGGUCGGCGCguucacgacgaaACUGg -3' miRNA: 3'- guCCAUAG---CUUGC-CCAGCCGCG------------UGAC- -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 10394 | 0.76 | 0.188502 |
Target: 5'- cCGGGccggccggCGAGCGGGUcCGGCGCGCg- -3' miRNA: 3'- -GUCCaua-----GCUUGCCCA-GCCGCGUGac -5' |
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24212 | 3' | -55.8 | NC_005263.2 | + | 10048 | 1.09 | 0.000809 |
Target: 5'- cCAGGUAUCGAACGGGUCGGCGCACUGg -3' miRNA: 3'- -GUCCAUAGCUUGCCCAGCCGCGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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