Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24223 | 3' | -54 | NC_005263.2 | + | 31511 | 0.66 | 0.705691 |
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Target: 5'- ----cUGCUCGCGCGCgagcccggcgaaCGCGUcgCCGc -3' miRNA: 3'- uaagaACGAGCGCGUGa-----------GCGCAa-GGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 40709 | 0.66 | 0.696765 |
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Target: 5'- ----cUGUUCgGCGCACUCGCGa-CCGc -3' miRNA: 3'- uaagaACGAG-CGCGUGAGCGCaaGGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 725 | 0.67 | 0.662958 |
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Target: 5'- uUUCaugUGCUCGCGCGCaUCGaaCGcgCCGc -3' miRNA: 3'- uAAGa--ACGAGCGCGUG-AGC--GCaaGGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 16595 | 0.67 | 0.662958 |
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Target: 5'- cGUUCgccggGCUCGCGCgcgagcagcaGCUCGCG--CCGc -3' miRNA: 3'- -UAAGaa---CGAGCGCG----------UGAGCGCaaGGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 45760 | 0.67 | 0.659557 |
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Target: 5'- cUUCcaGCU-GCGCaacggccgcacgguGCUCGCGUUCCGc -3' miRNA: 3'- uAAGaaCGAgCGCG--------------UGAGCGCAAGGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 8413 | 0.67 | 0.651612 |
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Target: 5'- uUUCcaGCUCGCGCucgccccaUCGCGU-CCAc -3' miRNA: 3'- uAAGaaCGAGCGCGug------AGCGCAaGGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 23077 | 0.68 | 0.606147 |
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Target: 5'- -gUCUUGCUgGCGCugUUgGCGUcgUUCGa -3' miRNA: 3'- uaAGAACGAgCGCGugAG-CGCA--AGGU- -5' |
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| 24223 | 3' | -54 | NC_005263.2 | + | 5193 | 1.07 | 0.001285 |
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Target: 5'- gAUUCUUGCUCGCGCACUCGCGUUCCAg -3' miRNA: 3'- -UAAGAACGAGCGCGUGAGCGCAAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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