Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24225 | 5' | -57.9 | NC_005263.2 | + | 29061 | 0.66 | 0.544424 |
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Target: 5'- -gCggCGCGuCCUCGG-GGCGGCg-- -3' miRNA: 3'- uaGaaGCGCuGGAGCCaCUGCCGguc -5' |
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| 24225 | 5' | -57.9 | NC_005263.2 | + | 20300 | 0.66 | 0.512774 |
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Target: 5'- -gCUUCGCGAacgCGaUGGCGGCCGc -3' miRNA: 3'- uaGAAGCGCUggaGCcACUGCCGGUc -5' |
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| 24225 | 5' | -57.9 | NC_005263.2 | + | 32895 | 0.67 | 0.492099 |
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Target: 5'- -gUUUCGUGGCgUCGGcgucGGCGGCCc- -3' miRNA: 3'- uaGAAGCGCUGgAGCCa---CUGCCGGuc -5' |
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| 24225 | 5' | -57.9 | NC_005263.2 | + | 17389 | 0.69 | 0.344279 |
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Target: 5'- uGUCUgccggCGCGGCCgcgcgccuggcgggCGGUGucgcaggugcaacaGCGGCCAGc -3' miRNA: 3'- -UAGAa----GCGCUGGa-------------GCCAC--------------UGCCGGUC- -5' |
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| 24225 | 5' | -57.9 | NC_005263.2 | + | 14157 | 0.79 | 0.078586 |
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Target: 5'- -cCUUgGCGACUUCGGgccGGCGGCCGGu -3' miRNA: 3'- uaGAAgCGCUGGAGCCa--CUGCCGGUC- -5' |
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| 24225 | 5' | -57.9 | NC_005263.2 | + | 3589 | 1.07 | 0.000602 |
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Target: 5'- gAUCUUCGCGACCUCGGUGACGGCCAGg -3' miRNA: 3'- -UAGAAGCGCUGGAGCCACUGCCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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