Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24237 | 5' | -49.8 | NC_005263.2 | + | 46069 | 0.67 | 0.900315 |
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Target: 5'- cGCG-CGGG-CGUCGAcgUgaucgaugcgGCCGUGa -3' miRNA: 3'- -CGCuGUCCaGCAGCUuaAa---------CGGCAUg -5' |
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| 24237 | 5' | -49.8 | NC_005263.2 | + | 3263 | 0.68 | 0.885411 |
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Target: 5'- cGCaGACAGG-CGUCGAGUaucGCCucGCa -3' miRNA: 3'- -CG-CUGUCCaGCAGCUUAaa-CGGcaUG- -5' |
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| 24237 | 5' | -49.8 | NC_005263.2 | + | 41173 | 0.71 | 0.742669 |
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Target: 5'- gGCGGCc-GUCGUCGAuacGUUgcgcGCUGUGCg -3' miRNA: 3'- -CGCUGucCAGCAGCU---UAAa---CGGCAUG- -5' |
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| 24237 | 5' | -49.8 | NC_005263.2 | + | 8914 | 0.72 | 0.672216 |
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Target: 5'- gGCGGCAGGaugUCGUCGAGgucggUgaucggcauaccgaUGCCGUcGCg -3' miRNA: 3'- -CGCUGUCC---AGCAGCUUa----A--------------ACGGCA-UG- -5' |
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| 24237 | 5' | -49.8 | NC_005263.2 | + | 13767 | 0.74 | 0.550265 |
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Target: 5'- gGCGACAGGcgCGUCGcuuAAUgggccgcaUGCUGUGCa -3' miRNA: 3'- -CGCUGUCCa-GCAGC---UUAa-------ACGGCAUG- -5' |
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| 24237 | 5' | -49.8 | NC_005263.2 | + | 4220 | 0.77 | 0.384501 |
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Target: 5'- uCGACGGGUCGUCGAGguagcgGCCuagcccGUGCu -3' miRNA: 3'- cGCUGUCCAGCAGCUUaaa---CGG------CAUG- -5' |
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| 24237 | 5' | -49.8 | NC_005263.2 | + | 20475 | 0.66 | 0.948112 |
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Target: 5'- aCGGCAacgccCGUCGAGguaaagGCCGUGCg -3' miRNA: 3'- cGCUGUcca--GCAGCUUaaa---CGGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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