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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24242 | 3' | -50.7 | NC_005263.2 | + | 29403 | 0.66 | 0.904135 |
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Target: 5'- aGggUUGCcgCCCGcgCCUgcCGCGc- -3' miRNA: 3'- aCuuAGCGuaGGGUuaGGAa-GCGCuc -5' |
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| 24242 | 3' | -50.7 | NC_005263.2 | + | 28862 | 0.66 | 0.889508 |
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Target: 5'- cGAGUCGUcgCgCCAGaagccggCUUUCGUGAGc -3' miRNA: 3'- aCUUAGCGuaG-GGUUa------GGAAGCGCUC- -5' |
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| 24242 | 3' | -50.7 | NC_005263.2 | + | 47539 | 0.66 | 0.881764 |
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Target: 5'- cGAucUCGCAUUCCAAggCCUgCGCGc- -3' miRNA: 3'- aCUu-AGCGUAGGGUUa-GGAaGCGCuc -5' |
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| 24242 | 3' | -50.7 | NC_005263.2 | + | 37342 | 0.67 | 0.865443 |
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Target: 5'- cGA--UGCGUUCCAGUCggUCGCGGc -3' miRNA: 3'- aCUuaGCGUAGGGUUAGgaAGCGCUc -5' |
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| 24242 | 3' | -50.7 | NC_005263.2 | + | 3774 | 0.68 | 0.80048 |
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Target: 5'- cGAcGUCGCGUUCUggUcggCCUUUGCGAu -3' miRNA: 3'- aCU-UAGCGUAGGGuuA---GGAAGCGCUc -5' |
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| 24242 | 3' | -50.7 | NC_005263.2 | + | 37461 | 0.69 | 0.769536 |
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Target: 5'- cGAAUCGCGUCUaCGA-CCUguacCGCGAu -3' miRNA: 3'- aCUUAGCGUAGG-GUUaGGAa---GCGCUc -5' |
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| 24242 | 3' | -50.7 | NC_005263.2 | + | 5500 | 1.1 | 0.002013 |
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Target: 5'- cUGAAUCGCAUCCCAAUCCUUCGCGAGg -3' miRNA: 3'- -ACUUAGCGUAGGGUUAGGAAGCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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