miRNA: 3'- aagucaagCGAGCGGUCCaGCUGCGC- -5'
miRNA: 3'- -AAGUCAAGCGAGCGGUCCAGCUGCGC- -5'
miRNA: 3'- aaGUCA-----AGCgAGCGGUcCAGCUGCGc -5'
miRNA: 3'- aaGUCaaGCGAGCGG------UCCAGC-UGCGc -5'
miRNA: 3'- -AAGUCa-------AGCGaGCGGUC----------CAGCUGCGc -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24253 | 5' | -56.9 | NC_005263.2 | + | 9566 | 0.68 | 0.479837 |
|
Target: 5'- -------gGCUUGCCAGG-CGGCGCGc -3' miRNA: 3'- aagucaagCGAGCGGUCCaGCUGCGC- -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 8372 | 1.07 | 0.000761 |
|
Target: 5'- gUUCAGUUCGCUCGCCAGGUCGACGCGg -3' miRNA: 3'- -AAGUCAAGCGAGCGGUCCAGCUGCGC- -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 5454 | 0.67 | 0.532084 |
|
Target: 5'- -gCAGUagcgaUCGgUUGCCAcGUCGGCGCu -3' miRNA: 3'- aaGUCA-----AGCgAGCGGUcCAGCUGCGc -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 2274 | 0.67 | 0.521456 |
|
Target: 5'- -gCAGcgCGUUCGCCgucggcAGGUCGuGCGCc -3' miRNA: 3'- aaGUCaaGCGAGCGG------UCCAGC-UGCGc -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 893 | 0.67 | 0.51091 |
|
Target: 5'- gUUCAGcgcggcggUCGC-CGCCGGcggguuacucGUCGACGCa -3' miRNA: 3'- -AAGUCa-------AGCGaGCGGUC----------CAGCUGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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