miRNA: 3'- aaguCA--AGCGaGCGGUC-CAGCUGCGc -5'
miRNA: 3'- aaGUCaa---GCGAGCGGUCC-AGC-UGCGC- -5'
miRNA: 3'- aAGUCaaGCG------AGCg-GUCCAGCUGCGc -5'
miRNA: 3'- aAGUCaagCGAGCGGUCCa-----GCUGCGC- -5'
miRNA: 3'- aagucaaGCGaGCGGUCCaGCUGCGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24253 | 5' | -56.9 | NC_005263.2 | + | 21582 | 0.68 | 0.430223 |
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Target: 5'- ---cGUgcUCGC-CGCCGGcGUCGACGCc -3' miRNA: 3'- aaguCA--AGCGaGCGGUC-CAGCUGCGc -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 16586 | 0.69 | 0.420666 |
|
Target: 5'- -gCGGcgacgCGUUCGCCGGGcUCGcGCGCGa -3' miRNA: 3'- aaGUCaa---GCGAGCGGUCC-AGC-UGCGC- -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 10039 | 0.69 | 0.420666 |
|
Target: 5'- gUCAGggCGCcagguaUCGaaCGGGUCGGCGCa -3' miRNA: 3'- aAGUCaaGCG------AGCg-GUCCAGCUGCGc -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 24790 | 0.72 | 0.265515 |
|
Target: 5'- gUCGGcg-GCUCGCCGGGcgucggCGGCGCGu -3' miRNA: 3'- aAGUCaagCGAGCGGUCCa-----GCUGCGC- -5' |
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| 24253 | 5' | -56.9 | NC_005263.2 | + | 46835 | 0.67 | 0.532084 |
|
Target: 5'- ------aCGCgCGCCAGG-CGGCGCGc -3' miRNA: 3'- aagucaaGCGaGCGGUCCaGCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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