miRNA: 3'- aguaCCuaCUGC-----CAGCACGCGUGGCG- -5'
miRNA: 3'- agUACCUA---CUGCCAGcACGCgUGGCG- -5'
miRNA: 3'- aGUACcuaCUGCcAGCACGCGUGGCG- -5'
miRNA: 3'- agUACCUACuGC--CAG-CACGCGUGGCG- -5'
miRNA: 3'- -AGUACCUACUGCCAGCACGCGUGGCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24267 | 3' | -55.3 | NC_005263.2 | + | 18280 | 0.72 | 0.334144 |
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Target: 5'- ---cGGgcGAUGcgcucGUCGUGCGCGCUGCa -3' miRNA: 3'- aguaCCuaCUGC-----CAGCACGCGUGGCG- -5' |
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| 24267 | 3' | -55.3 | NC_005263.2 | + | 26175 | 0.72 | 0.334144 |
|
Target: 5'- -gGUGGAUgagGACGGUC-UGCG-GCCGCg -3' miRNA: 3'- agUACCUA---CUGCCAGcACGCgUGGCG- -5' |
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| 24267 | 3' | -55.3 | NC_005263.2 | + | 7032 | 0.73 | 0.295337 |
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Target: 5'- aCGUGuc-GACGaUCGUGCGCGCCGUc -3' miRNA: 3'- aGUACcuaCUGCcAGCACGCGUGGCG- -5' |
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| 24267 | 3' | -55.3 | NC_005263.2 | + | 38346 | 0.79 | 0.118582 |
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Target: 5'- -gAUGGAUG-CGaaGUCgGUGCGCACCGCg -3' miRNA: 3'- agUACCUACuGC--CAG-CACGCGUGGCG- -5' |
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| 24267 | 3' | -55.3 | NC_005263.2 | + | 13646 | 1.13 | 0.000476 |
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Target: 5'- aUCAUGGAUGACGGUCGUGCGCACCGCg -3' miRNA: 3'- -AGUACCUACUGCCAGCACGCGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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