Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24280 | 5' | -55.3 | NC_005263.2 | + | 28529 | 0.66 | 0.646553 |
Target: 5'- cUUCGguUCGACGcCGgGCUCGAGCu--- -3' miRNA: 3'- -AAGC--AGCUGUaGCgCGAGCUCGucaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 30804 | 0.66 | 0.646553 |
Target: 5'- cUCGgCGGCGaCGUGCUCG-GCGGa- -3' miRNA: 3'- aAGCaGCUGUaGCGCGAGCuCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 47342 | 0.66 | 0.654452 |
Target: 5'- --aGUCGuACAUCGCGCUgcgcgucgccgaaaUGGGCGGc- -3' miRNA: 3'- aagCAGC-UGUAGCGCGA--------------GCUCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 10207 | 0.66 | 0.657835 |
Target: 5'- cUCGcCGGCcUCGgGCUCGAGUccAGa- -3' miRNA: 3'- aAGCaGCUGuAGCgCGAGCUCG--UCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 17008 | 0.66 | 0.657835 |
Target: 5'- -gUGUCGAagcguUCGUaucGCUCGAGCAGc- -3' miRNA: 3'- aaGCAGCUgu---AGCG---CGAGCUCGUCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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