miRNA: 3'- gAGUAGG-UGGUCCUGU-UGCUGcGUCC- -5'
miRNA: 3'- gaGUaGGUGGuCCUGUUGCUGCG--UCC- -5'
miRNA: 3'- gaguaGGUGGUCCUGUUGCUgCGUCC- -5'
miRNA: 3'- -GAGUAGGUGGuCCUGUUGCUGCGUCC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24284 | 5' | -53.6 | NC_005263.2 | + | 1246 | 0.71 | 0.484262 |
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Target: 5'- gUCAUCCgGCCAGGAUGcGCGAUcCGGGu -3' miRNA: 3'- gAGUAGG-UGGUCCUGU-UGCUGcGUCC- -5' |
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| 24284 | 5' | -53.6 | NC_005263.2 | + | 16557 | 0.75 | 0.295517 |
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Target: 5'- -gCG-CCGCCuGGGCAGCGACGCcaAGGc -3' miRNA: 3'- gaGUaGGUGGuCCUGUUGCUGCG--UCC- -5' |
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| 24284 | 5' | -53.6 | NC_005263.2 | + | 485 | 0.78 | 0.193978 |
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Target: 5'- ----gCCGCCAGGACGAUGA-GCAGGa -3' miRNA: 3'- gaguaGGUGGUCCUGUUGCUgCGUCC- -5' |
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| 24284 | 5' | -53.6 | NC_005263.2 | + | 21946 | 1 | 0.00638 |
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Target: 5'- cCUCAUCCACC-GGACAACGACGCAGGc -3' miRNA: 3'- -GAGUAGGUGGuCCUGUUGCUGCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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