Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24293 | 5' | -55 | NC_005263.2 | + | 11958 | 0.66 | 0.650051 |
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Target: 5'- uUCGCCUcgagCgcgcGCCGuacuugcgcucGAUCGAGCGCAAg -3' miRNA: 3'- cAGUGGAa---Ga---CGGC-----------CUAGCUCGUGUU- -5' |
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| 24293 | 5' | -55 | NC_005263.2 | + | 3736 | 0.66 | 0.638726 |
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Target: 5'- -aCGCCgg--GCCGGAUC-AGCGCGu -3' miRNA: 3'- caGUGGaagaCGGCCUAGcUCGUGUu -5' |
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| 24293 | 5' | -55 | NC_005263.2 | + | 27104 | 0.67 | 0.604753 |
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Target: 5'- aUCGCCUgaaacGCCGGcacGUCGAcGCGCAGg -3' miRNA: 3'- cAGUGGAaga--CGGCC---UAGCU-CGUGUU- -5' |
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| 24293 | 5' | -55 | NC_005263.2 | + | 36184 | 0.67 | 0.559847 |
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Target: 5'- --gGCCggCUGCCGGAUaucGCACAGg -3' miRNA: 3'- cagUGGaaGACGGCCUAgcuCGUGUU- -5' |
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| 24293 | 5' | -55 | NC_005263.2 | + | 43081 | 0.69 | 0.463094 |
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Target: 5'- -cCGCCgg--GCCGGcuGUCGAGCGCGg -3' miRNA: 3'- caGUGGaagaCGGCC--UAGCUCGUGUu -5' |
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| 24293 | 5' | -55 | NC_005263.2 | + | 27464 | 1.05 | 0.001312 |
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Target: 5'- uGUCACCUUCUGCCGGAUCGAGCACAAc -3' miRNA: 3'- -CAGUGGAAGACGGCCUAGCUCGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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