Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24296 | 3' | -55.4 | NC_005263.2 | + | 34916 | 0.66 | 0.670017 |
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Target: 5'- cGAAUgucgACCUgUGAUGCGCGCGgaGCUGa -3' miRNA: 3'- -CUUG----UGGA-GUUGCGCGCGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 20352 | 0.66 | 0.670017 |
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Target: 5'- -cGCACCUCgGGgGCGCGCaugGCUGa -3' miRNA: 3'- cuUGUGGAG-UUgCGCGCGcaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 16572 | 0.66 | 0.658908 |
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Target: 5'- cGACGCCaaggcCGGCgGCGaCGCGUUcGCCGg -3' miRNA: 3'- cUUGUGGa----GUUG-CGC-GCGCAA-CGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 35986 | 0.66 | 0.647773 |
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Target: 5'- gGAGCGCgCguaAugGCGCGCGc-GCCGc -3' miRNA: 3'- -CUUGUG-Gag-UugCGCGCGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 10658 | 0.66 | 0.647773 |
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Target: 5'- uGGACGCCgcgCAGacCGCGCGC---GCCGa -3' miRNA: 3'- -CUUGUGGa--GUU--GCGCGCGcaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 3340 | 0.66 | 0.636623 |
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Target: 5'- -cGCGCCgcCGGCGcCGCGCGcgGUCGa -3' miRNA: 3'- cuUGUGGa-GUUGC-GCGCGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 34050 | 0.66 | 0.625469 |
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Target: 5'- cGGCGCgCUCAAUGCGCucgacGCGgcGcCCGUg -3' miRNA: 3'- cUUGUG-GAGUUGCGCG-----CGCaaC-GGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 16988 | 0.66 | 0.68109 |
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Target: 5'- cGAAUACCUgAGCGaCGCGacc-GCCGa -3' miRNA: 3'- -CUUGUGGAgUUGC-GCGCgcaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 9700 | 0.66 | 0.68109 |
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Target: 5'- -cGCACC-CGACGCGCGacaGUgcGUCGa -3' miRNA: 3'- cuUGUGGaGUUGCGCGCg--CAa-CGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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