Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24296 | 3' | -55.4 | NC_005263.2 | + | 43499 | 0.67 | 0.614321 |
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Target: 5'- cGGCACCUCGAagcUGCcgGCGCG--GCCGUu -3' miRNA: 3'- cUUGUGGAGUU---GCG--CGCGCaaCGGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 43852 | 0.68 | 0.505176 |
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Target: 5'- cGGGCGCaaCAACGUGCGCGgcauccugacGCCGa -3' miRNA: 3'- -CUUGUGgaGUUGCGCGCGCaa--------CGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 44123 | 0.68 | 0.559006 |
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Target: 5'- -cGCugCUCGGCgGCGCGCGc--CCGUu -3' miRNA: 3'- cuUGugGAGUUG-CGCGCGCaacGGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 44388 | 0.69 | 0.463772 |
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Target: 5'- uGGACAUgcguaUCAACGCGCGCGgcaUUGgCGUc -3' miRNA: 3'- -CUUGUGg----AGUUGCGCGCGC---AACgGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 44655 | 0.76 | 0.18877 |
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Target: 5'- -uGCGCCUCGAUGCGuCGCGcgcGCCGa -3' miRNA: 3'- cuUGUGGAGUUGCGC-GCGCaa-CGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 44749 | 0.71 | 0.38674 |
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Target: 5'- -cGCACugucuggucgCUCGAcCGCGCGCGgcGCCGg -3' miRNA: 3'- cuUGUG----------GAGUU-GCGCGCGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 45458 | 0.69 | 0.484262 |
|
Target: 5'- -uGCACCUCGAgccCG-GCGCGaacgUGCCGa -3' miRNA: 3'- cuUGUGGAGUU---GCgCGCGCa---ACGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 45796 | 0.72 | 0.334777 |
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Target: 5'- --cCGCCaguUCAACGCGgGCG-UGCCGg -3' miRNA: 3'- cuuGUGG---AGUUGCGCgCGCaACGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 47132 | 0.66 | 0.647773 |
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Target: 5'- aAGCGCCgucCGACGCGCagGCGaccGCCGc -3' miRNA: 3'- cUUGUGGa--GUUGCGCG--CGCaa-CGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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