Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24296 | 3' | -55.4 | NC_005263.2 | + | 4848 | 0.68 | 0.526469 |
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Target: 5'- ---uGCC-CGuACGCGCGCGccgUGCCGa -3' miRNA: 3'- cuugUGGaGU-UGCGCGCGCa--ACGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 4462 | 0.68 | 0.559006 |
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Target: 5'- cGAGCACUUCuucguccguGCGCGCGgGUcGgCGUg -3' miRNA: 3'- -CUUGUGGAGu--------UGCGCGCgCAaCgGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 3940 | 0.66 | 0.68109 |
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Target: 5'- uAGCGCCUCGcACGCGCcagccaGCGagccuggcagGCCGUu -3' miRNA: 3'- cUUGUGGAGU-UGCGCG------CGCaa--------CGGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 3722 | 0.77 | 0.15138 |
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Target: 5'- uGAGCGCCUCGcgcACGCcgggccggaucaGCGCGUacgUGCCGUa -3' miRNA: 3'- -CUUGUGGAGU---UGCG------------CGCGCA---ACGGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 3708 | 0.66 | 0.68109 |
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Target: 5'- cGACGCCaaugcCGCGCGCGUUGauaCGc -3' miRNA: 3'- cUUGUGGaguu-GCGCGCGCAACg--GCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 3340 | 0.66 | 0.636623 |
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Target: 5'- -cGCGCCgcCGGCGcCGCGCGcgGUCGa -3' miRNA: 3'- cuUGUGGa-GUUGC-GCGCGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 2263 | 0.66 | 0.647773 |
|
Target: 5'- --gUugUUCgAGCGCaGCGCGUUcGCCGUc -3' miRNA: 3'- cuuGugGAG-UUGCG-CGCGCAA-CGGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 1614 | 0.69 | 0.453698 |
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Target: 5'- cGGACACUUCGACGCcgaGUGCGUcgGUCa- -3' miRNA: 3'- -CUUGUGGAGUUGCG---CGCGCAa-CGGca -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 670 | 0.81 | 0.08079 |
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Target: 5'- cGAACGCCUCAucguaGCGCGCcUUGCCGa -3' miRNA: 3'- -CUUGUGGAGUug---CGCGCGcAACGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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