Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24296 | 3' | -55.4 | NC_005263.2 | + | 18861 | 0.68 | 0.547002 |
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Target: 5'- uGAACaacgcguGCCUCGGCGCGCcgcuGCGc-GCCGa -3' miRNA: 3'- -CUUG-------UGGAGUUGCGCG----CGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 8989 | 0.68 | 0.547002 |
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Target: 5'- cGAGCGCCgacgcaCGGCGCacgagucGCGCGUacucGCCGUc -3' miRNA: 3'- -CUUGUGGa-----GUUGCG-------CGCGCAa---CGGCA- -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 25716 | 0.68 | 0.537242 |
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Target: 5'- uGGGCGCCUCuuuccuGGCGCuGCGCGccgUGCaCGa -3' miRNA: 3'- -CUUGUGGAG------UUGCG-CGCGCa--ACG-GCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 24686 | 0.68 | 0.526469 |
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Target: 5'- gGGGCGCCgguaacggCGGCGCGC-CGaacgUGCCGg -3' miRNA: 3'- -CUUGUGGa-------GUUGCGCGcGCa---ACGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 5251 | 0.68 | 0.526469 |
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Target: 5'- aGGCGCCgUCAucaGCGgGCGgUGCCGg -3' miRNA: 3'- cUUGUGG-AGUug-CGCgCGCaACGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 43852 | 0.68 | 0.505176 |
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Target: 5'- cGGGCGCaaCAACGUGCGCGgcauccugacGCCGa -3' miRNA: 3'- -CUUGUGgaGUUGCGCGCGCaa--------CGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 27118 | 0.69 | 0.463772 |
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Target: 5'- cGGCACgUCGACGCGCaggcGCGcaGCCGc -3' miRNA: 3'- cUUGUGgAGUUGCGCG----CGCaaCGGCa -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 1614 | 0.69 | 0.453698 |
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Target: 5'- cGGACACUUCGACGCcgaGUGCGUcgGUCa- -3' miRNA: 3'- -CUUGUGGAGUUGCG---CGCGCAa-CGGca -5' |
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| 24296 | 3' | -55.4 | NC_005263.2 | + | 670 | 0.81 | 0.08079 |
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Target: 5'- cGAACGCCUCAucguaGCGCGCcUUGCCGa -3' miRNA: 3'- -CUUGUGGAGUug---CGCGCGcAACGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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