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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24304 | 5' | -51.7 | NC_005263.2 | + | 10920 | 0.66 | 0.84232 |
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Target: 5'- gGGGGAUGCCg-CACGUga-UCGCgUCg -3' miRNA: 3'- aUUCCUGCGGaaGUGCAaucAGCG-AG- -5' |
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| 24304 | 5' | -51.7 | NC_005263.2 | + | 6202 | 0.68 | 0.751758 |
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Target: 5'- -cAGGAaGCCggUCGCGUUGGUCuuCUCc -3' miRNA: 3'- auUCCUgCGGa-AGUGCAAUCAGc-GAG- -5' |
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| 24304 | 5' | -51.7 | NC_005263.2 | + | 4252 | 0.69 | 0.650419 |
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Target: 5'- -cAGGAUGCCgcgCACGUU-GUUGCg- -3' miRNA: 3'- auUCCUGCGGaa-GUGCAAuCAGCGag -5' |
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| 24304 | 5' | -51.7 | NC_005263.2 | + | 30151 | 0.7 | 0.627377 |
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Target: 5'- --uGGAUGCCgUUCACGagcuggcgcgGGUCGUUCg -3' miRNA: 3'- auuCCUGCGG-AAGUGCaa--------UCAGCGAG- -5' |
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| 24304 | 5' | -51.7 | NC_005263.2 | + | 13966 | 0.7 | 0.581434 |
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Target: 5'- --cGGcGCGCgUUCGCGgggcgugUAGUUGCUCa -3' miRNA: 3'- auuCC-UGCGgAAGUGCa------AUCAGCGAG- -5' |
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| 24304 | 5' | -51.7 | NC_005263.2 | + | 40882 | 0.74 | 0.372144 |
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Target: 5'- aGAGGcgcGCGCCUUCugGUcgccggcgaUGGUCGCg- -3' miRNA: 3'- aUUCC---UGCGGAAGugCA---------AUCAGCGag -5' |
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| 24304 | 5' | -51.7 | NC_005263.2 | + | 33622 | 1.09 | 0.001603 |
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Target: 5'- aUAAGGACGCCUUCACGUUAGUCGCUCg -3' miRNA: 3'- -AUUCCUGCGGAAGUGCAAUCAGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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