Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24312 | 5' | -52.8 | NC_005263.2 | + | 35887 | 0.66 | 0.825271 |
Target: 5'- --uUCGCCGCgUUgAUGUCgGUGcggUCGa -3' miRNA: 3'- guuAGCGGCG-AAgUACGGgCACa--AGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 1166 | 0.66 | 0.805139 |
Target: 5'- cCGAUCGCCucggcuuGCUUCGggcucuUGCCCGc-UUCGa -3' miRNA: 3'- -GUUAGCGG-------CGAAGU------ACGGGCacAAGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 38653 | 0.66 | 0.786194 |
Target: 5'- gGcgCGCCGCUgcccgUgGUGCCCGUcuuGaUCGg -3' miRNA: 3'- gUuaGCGGCGA-----AgUACGGGCA---CaAGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 28435 | 0.67 | 0.765595 |
Target: 5'- -cAUCGCUgaGCUUCAccgGCCCGgcaUUCGa -3' miRNA: 3'- guUAGCGG--CGAAGUa--CGGGCac-AAGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 9884 | 0.67 | 0.755072 |
Target: 5'- --cUCGgCGCuUUCAUGCUCGUcgUCGg -3' miRNA: 3'- guuAGCgGCG-AAGUACGGGCAcaAGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 20659 | 0.67 | 0.733642 |
Target: 5'- ---gCGCCGCUcgUCGcgGCCgucgaaagCGUGUUCGg -3' miRNA: 3'- guuaGCGGCGA--AGUa-CGG--------GCACAAGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 23775 | 0.67 | 0.722759 |
Target: 5'- gGcgCGCCGUUUUgcggaauugcgAUGCCCGUGccggCGg -3' miRNA: 3'- gUuaGCGGCGAAG-----------UACGGGCACaa--GC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 46779 | 0.68 | 0.68959 |
Target: 5'- ----gGCCGUgaUCGUGUUCGUGUUCa -3' miRNA: 3'- guuagCGGCGa-AGUACGGGCACAAGc -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 2282 | 0.69 | 0.644601 |
Target: 5'- --uUCGCCGUcggcaggUCGUGCgCCGUGgcCGg -3' miRNA: 3'- guuAGCGGCGa------AGUACG-GGCACaaGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 20549 | 0.69 | 0.633295 |
Target: 5'- aCAAccUCGCCGCgacgcUCGUGCCCGg----- -3' miRNA: 3'- -GUU--AGCGGCGa----AGUACGGGCacaagc -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 2754 | 0.72 | 0.468804 |
Target: 5'- aGAUCGCgGCcgcCGUGCCgguCGUGUUCGu -3' miRNA: 3'- gUUAGCGgCGaa-GUACGG---GCACAAGC- -5' |
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24312 | 5' | -52.8 | NC_005263.2 | + | 38992 | 1.08 | 0.001676 |
Target: 5'- gCAAUCGCCGCUUCAUGCCCGUGUUCGu -3' miRNA: 3'- -GUUAGCGGCGAAGUACGGGCACAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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