Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24313 | 5' | -54 | NC_005263.2 | + | 29514 | 0.67 | 0.686023 |
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Target: 5'- uGCGGCGUCCgucgGCgCGCCAGcagugGCc -3' miRNA: 3'- cUGCUGUAGGa---CG-GCGGUCauaa-CGc -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 14996 | 0.67 | 0.686023 |
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Target: 5'- -gUGACGUUCUGCuCGCCGGcUAUcGCc -3' miRNA: 3'- cuGCUGUAGGACG-GCGGUC-AUAaCGc -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 45436 | 0.67 | 0.674851 |
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Target: 5'- cGCGACA-CUgcagGCUGCCGGUGUcGCc -3' miRNA: 3'- cUGCUGUaGGa---CGGCGGUCAUAaCGc -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 4331 | 0.67 | 0.663635 |
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Target: 5'- aACGGCccgauaaguuuAUCgaGCCGUUAGUGUUGUGu -3' miRNA: 3'- cUGCUG-----------UAGgaCGGCGGUCAUAACGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 6444 | 0.67 | 0.641123 |
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Target: 5'- -cCGACGaUCUGCCGCCAGagcacgaGCGa -3' miRNA: 3'- cuGCUGUaGGACGGCGGUCauaa---CGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 7349 | 0.68 | 0.618577 |
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Target: 5'- aGCGACuugCC-GCCGCCGGUGacgaucGCGg -3' miRNA: 3'- cUGCUGua-GGaCGGCGGUCAUaa----CGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 39342 | 0.71 | 0.44581 |
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Target: 5'- cGGCGuCAUCCUGCCGCgCAGcGUUa-- -3' miRNA: 3'- -CUGCuGUAGGACGGCG-GUCaUAAcgc -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 7286 | 0.71 | 0.435848 |
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Target: 5'- aGCGACAUgacgcgugCCUGCgGCCAGgucUGCGc -3' miRNA: 3'- cUGCUGUA--------GGACGgCGGUCauaACGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 24783 | 0.66 | 0.740835 |
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Target: 5'- gGGCGGCGUCggcgGCuCGCCGGgcgucgGCGg -3' miRNA: 3'- -CUGCUGUAGga--CG-GCGGUCauaa--CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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