Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24313 | 5' | -54 | NC_005263.2 | + | 39342 | 0.71 | 0.44581 |
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Target: 5'- cGGCGuCAUCCUGCCGCgCAGcGUUa-- -3' miRNA: 3'- -CUGCuGUAGGACGGCG-GUCaUAAcgc -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 7286 | 0.71 | 0.435848 |
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Target: 5'- aGCGACAUgacgcgugCCUGCgGCCAGgucUGCGc -3' miRNA: 3'- cUGCUGUA--------GGACGgCGGUCauaACGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 30297 | 0.71 | 0.426016 |
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Target: 5'- cGCGGCAUCCUcgGCCGCCuugccaUGCGc -3' miRNA: 3'- cUGCUGUAGGA--CGGCGGucaua-ACGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 36319 | 0.71 | 0.416316 |
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Target: 5'- cGCGACGUCCUG-CGCCA----UGCGa -3' miRNA: 3'- cUGCUGUAGGACgGCGGUcauaACGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 44506 | 0.72 | 0.397329 |
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Target: 5'- cGGCGGCAUCCUgGCCGUCAccgagGUcGCGa -3' miRNA: 3'- -CUGCUGUAGGA-CGGCGGUca---UAaCGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 30357 | 0.73 | 0.35237 |
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Target: 5'- cGCGGCcgCgCUGCCGCCGGcgccgagUGCGa -3' miRNA: 3'- cUGCUGuaG-GACGGCGGUCaua----ACGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 26949 | 0.76 | 0.215472 |
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Target: 5'- uGAUGACGUCgCUGCCGUCGGUcgUGa- -3' miRNA: 3'- -CUGCUGUAG-GACGGCGGUCAuaACgc -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 12498 | 0.77 | 0.193195 |
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Target: 5'- uGCGGCAUCCUGCgCGCCGGcuucgcGCGg -3' miRNA: 3'- cUGCUGUAGGACG-GCGGUCauaa--CGC- -5' |
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| 24313 | 5' | -54 | NC_005263.2 | + | 39200 | 1.08 | 0.001171 |
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Target: 5'- cGACGACAUCCUGCCGCCAGUAUUGCGc -3' miRNA: 3'- -CUGCUGUAGGACGGCGGUCAUAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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