Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24320 | 5' | -55.2 | NC_005263.2 | + | 36680 | 0.66 | 0.661706 |
Target: 5'- cCUGGcAGCauuCGA--GCGCGGGCAAGGAg -3' miRNA: 3'- -GACC-UUGc--GCUcaCGCGCUCGUUCUU- -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 15313 | 0.66 | 0.660581 |
Target: 5'- -aGcGAgcuGCGCGAG-GCGCGGGCcgacaccGAGAAc -3' miRNA: 3'- gaC-CU---UGCGCUCaCGCGCUCG-------UUCUU- -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 47371 | 0.66 | 0.650448 |
Target: 5'- aUGGGcgGCGCGuucgauGcGCGCGAGCAcauGAAa -3' miRNA: 3'- gACCU--UGCGCu-----CaCGCGCUCGUu--CUU- -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 41691 | 0.66 | 0.61661 |
Target: 5'- -cGGAcUGCGGccGUaCGCGGGCAAGGAg -3' miRNA: 3'- gaCCUuGCGCU--CAcGCGCUCGUUCUU- -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 19851 | 0.67 | 0.594104 |
Target: 5'- gCUGcAGCGCGugcaGGUGCGCGAcucGCAGGu- -3' miRNA: 3'- -GACcUUGCGC----UCACGCGCU---CGUUCuu -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 30440 | 0.67 | 0.582898 |
Target: 5'- --cGAGCGCGAGcaGCGUGAGCGcGGc -3' miRNA: 3'- gacCUUGCGCUCa-CGCGCUCGUuCUu -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 33958 | 0.68 | 0.538588 |
Target: 5'- aCUGcGGGCcgGCGAGUGUGCGgcgaaucguagcGGCGAGGc -3' miRNA: 3'- -GAC-CUUG--CGCUCACGCGC------------UCGUUCUu -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 39687 | 0.69 | 0.484952 |
Target: 5'- gUGG-ACGCGAuGgggcgaGCGCGAGCuGGAAa -3' miRNA: 3'- gACCuUGCGCU-Ca-----CGCGCUCGuUCUU- -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 8856 | 0.69 | 0.464213 |
Target: 5'- -cGGcGCGCGGG-GCGCGAGCc---- -3' miRNA: 3'- gaCCuUGCGCUCaCGCGCUCGuucuu -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 21191 | 0.69 | 0.454018 |
Target: 5'- -cGGAcGCGCGcG-GCGCGGGCGAGu- -3' miRNA: 3'- gaCCU-UGCGCuCaCGCGCUCGUUCuu -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 2333 | 0.69 | 0.450983 |
Target: 5'- -cGGAACGCGAGcaccgugcggccguUGCGC-AGCuGGAAg -3' miRNA: 3'- gaCCUUGCGCUC--------------ACGCGcUCGuUCUU- -5' |
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24320 | 5' | -55.2 | NC_005263.2 | + | 42910 | 0.98 | 0.004892 |
Target: 5'- cCUGG-ACGCGAGUGCGCGAGCAAGAAu -3' miRNA: 3'- -GACCuUGCGCUCACGCGCUCGUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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